1. (on the Wecoma)

2. Wecoma Cruise Schedule

3. Wecoma Cruise Schedule (week 1)

4. Wecoma Cruise Schedule (week 2)

5. Wecoma Cruise Schedule (week 3)

6. Wecoma Cruise Coastal Lines Water samples Sediment samples CTD profiles (all stations) * Sediment samples can also be collected on Strawberry Hill and Cape Meares lines

7. Water sample analyses DNA & RNA samples (30 L per sample) –Community fingerprinting –Transcriptomics CARD-FISH filter preparation (0.05 L) Chemistry (5 L) –Dissolved Inorganic Nutrients: NO 3 +NO 2, NO 2, NH 4, PO 4 –Dissolved Organic Carbon –Particulate Organic Carbon and Nitrogen –Chlorophyll a –Suspended Particle Mass in estuary and plume only Bacterial Production (0.1 L) Bacterial and Total Respiration (5 L) Bacterial Abundance (0.02)

8. Sediment analyses Community fingerprinting Transcriptomics Membrane lipid analysis Characterization of geochemical gradients

9. Wecoma Cruise Scientific Personnel

10. Loading: Aug 13 Mooring equipment (Murray Levine, Walt Waldorf, Craig Risien, Wet Labs person) Coring equipment (Dale Hubbard) CTD setup & sensor installation (Dave O’Gorman, Sonia Newell, Michael Wilkin) DNA/RNA filtering station (Daniel Murphy, Carolyn Sheehan, MAYBE NEED PERSON?) Nutrients, POC/N, chlorophyll filtering stations (Carolyn Fortunato, NEED PERSON) Bacterial production & respiration stations (Jude Apple)

11. Leg 1: Aug 14-15 (mooring) Mooring placement (Murray Levine, Walt Waldorf, Craig Risien, Wet Labs person) CTD daytime (Dave O’Gorman, NEED PERSON) CTD nighttime (Sonya Newell, NEED PERSON) Practice water sample collection and processing –Practice DNA/RNA processing (Daniel Murphy, Carolyn Sheehan, MAYBE NEED PERSON?) –Practice POC/N filtration, nutrients (Carolyn Fortunato) –Practice chlorophyll filtrations (NEED PERSON) –Practice bacterial productions and respirations (Jude Apple) 0 empty berths

12. Leg 2: Aug 15-23 (Coast & plume) CTD daytime (Dave O’Gorman, NEED PERSON) CTD nighttime (Sonya Newell, NEED PERSON) Water sample collection (everyone) DNA/RNA processing (Daniel Murphy, Carolyn Sheehan, MAYBE NEED PERSON?) POC/N filtration, nutrients (Carolyn Fortunato) Chlorophyll filtrations (NEED PERSON) Bacterial production and respiration (Jude Apple) 2 empty berths

13. Leg 3: Aug 23-31 (Estuary & plume) CTD daytime (Dave O’Gorman, NEED PERSON) CTD nighttime (Sonya Newell, NEED PERSON) Water sample collection (everyone) DNA/RNA processing (NEED PERSON, Carolyn Sheehan, MAYBE NEED PERSON?) POC/N filtration, nutrients (Carolyn Fortunato) Chlorophyll filtrations (NEED PERSON) Bacterial productions and respirations (Joanna Green) 4 empty berths

14. Issue 1: Post-Cruise Need Shore-side Assistance with sample transport - someone to meet the ship –DNA/RNA samples - Transport to OHSU in coolers of dry ice. –POC/N, Chlorophyll, TDN/TDP, DOC, CARD-FISH, SPM - Transport to OHSU in coolers of dry ice. –Nutrient samples - Transport to OSU in coolers of dry ice. Shipping to Maryland - need to figure out how to do this Radioisotope - OSU rad safety will meet ship and mail samples to Maryland.

15. Issue 2: Communications E-mail with shipboard address via Quick pidgeon messenger service Internet - expensive, discouraged Ship-to-ship communication? Telephone? Cell Phones?

16. Issue 3: Limited Expertise Scientific Leadership –1 PI (Crump) –1 post-doc (Apple, 1/2 of cruise) Technical expertise –CTD?

17. Issue 4: Site selection Use Corie output to pre-select sampling stations Coastal sites - span upwelling fronts or other transition zones Plume - span the plume front (2-4 times in 2 different 2-day periods Estuary - sample surface waters at specific salinities, identify with surface flow-through system

18. Issue 5: Estuary operations Estuary operations (3 days) –Goal - 3 river-ocean transects across salinity gradient while Barnes samples ETMs –10 to 15 samples per transect –Only sample during day –Cannot sample at peak tides Can we predict the salinity field in the estuary to estimate when and where to sample? What do we do at night?

19. Cruise goals Mooring placement at station NH-10 along the Newport Hydroline. Occupy a grid of stations over the Oregon and Washington continental shelf and slope to map hydrographic, bio-optical, biological and chemical parameters. –Identify and characterize gradients –Relate gradients to patterns in microbial activity, community composition, and genetic expression –Combine these data with ADCP measurements of subsurface velocity to explore biophysical interactions. Make continuous measurements of surface water chemistry for higher resolution mapping.

20. Micro cruise goals Collect water samples for DNA- and RNA-based microbial community analyses across environmental gradients in pelagic environments of the Columbia River estuary, the Columbia River plume, and along established sampling lines along the Oregon and Washington coasts. Collect sediment samples with the multicorer at select sites along the Newport Hydroline and the Columbia River Line to characterize surface sediment microbial groups of special interest such as Crenarchaeota or Anammox bacteria.

21. Questions How are planktonic & benthic microbial diversity and activity distributed across river- estuary-coastal ecosystems? What gradients influence microbial diversity, activity & gene expression? How does diversity relate to temporal stability in an environment? (i.e., at what point does a transition zone become a stable environment?)

22. Goals Use nowcast/forecast system to locate environmental gradients and select sampling sites. Use ship to ship communication to coordinate ETM sampling.

23. Coastal Margin Science: Microbial Communities in Productive Coastal Margins Determine how microbial populations form across the physical and chemical gradients of the Columbia River river-to-ocean system and to uncover the microbial-mediated activities therein. Central theme of cruises Too many samples? Cruise too long?

24. Coastal Margin Science: Ecosystem Dynamics, Climate and Water Use Describe physical characteristics of the estuary and plume –Are these cruises contributing to this goal? Provide guidance for microbial sampling –In the works Ecosystem dynamics, biological productivity, air-sea carbon fluxes –Not well addressed. No surface mapping of O2, CO2, and pigments. No primary production. No zooplankton sampling.

25. Enabling Technologies: Sensors No sensors being tested (yet) Nucleic acid hybridization array –Cruise provides data for array development Airborne remote sensing –Collecting data during cruise

26. Enabling Technologies: Information and Visualization