Presentation on theme: "CO2 Diffusion Through Gelatin Experiment"— Presentation transcript:
1 CO2 Diffusion Through Gelatin Experiment Presented byJennifer Welborn June 2012
2 Learning Goals..Through this PowerPoint, STEMDIGITAL participants will:See how the concept of diffusion is related to a variety of curricular standardsUnderstand how ADI software can be used to show the process and rate of diffusion both qualitatively and quantitatively
3 OVERVIEW Multidiscipline Standards Alignment Concept Development Factors Affecting Rate of DiffusionDiffusion and Global Environmental ChangeResearch QuestionsExperiment--Diffusion of CO2 Through Gelatin: Model of Diffusion in CellsUsing the Digital Camera as a Data Collection ToolAnalyzing Data Using ADI Software
4 Standards AlignmentPhysical Science/Chemistry: particle motion theory; pH; temperature; mixtures and solutions; color change as an indicator of a change in physical properties/chemical composition; acid/base indicators and protonationBiology: passive transport; cellular structure, etc.Ecology/Environmental Science: environmental effects on living cells/systemsMath: rates; relationships; data collection, organization, analysis, interpretation, ratios and proportions
5 DiffusionDiffusion– movement of a substance from a region of higher concentration to a region of lower concentration.Diffusion continues until equilibrium--- the concentration of a substance is equal throughout a space
6 Carbon dioxide from the environment diffuses into plant cells Diffusion and CellsDissolved particles that are small or non-polar can diffuse through thecell membranes.The process of diffusion is one of the ways in which substances like oxygen, carbon dioxide and water move into and out of cells.Carbon dioxide from the environment diffuses into plant cells
7 Factors That Affect Rate of Diffusion TemperatureConcentration of material diffusingDensity of substance material is diffusing intoParticle SizeDiffusion and Global Environmental ChangeWhat might be the impact of increasing temperatures andatmospheric CO2 levels on diffusion in cells?
8 Research QuestionsCan a digital camera be used to quantify a change in thecolor of gelatin with BTB in it as carbon dioxidediffuses through it over time?What affect might an increase in the concentration of CO2have on the rate of CO2 diffusion through the gelatin?What effect might an increase in temperature have on therate of diffusion?How might this model apply to living organisms and changesin the environment?
9 Conducting the Experiment/Gathering and Analyzing Data Set up the experiment according to the directions on the lab directions handout.Photograph the control at the beginning, then a sample of each cube exposed to CO2 over time (1 hour, 6 hours).Use the ADI line tool, R, G, B values and graph colors option (SPACIAL ANALYSIS ) to analyze data both qualitatively and quantitatively.
16 Draw a line using a color of choice Click these to fine tunePlacement of starting and ending pointsNote beginning and ending coordinatesNote R,G, B valuesDraw a line using a color of choiceZoom in to help youPlace the beginning and ending points
20 The Value of The Control The color graph for the control offers a baseline of R, G, B values to the student.The next set of slides shows a sample of gelatin exposed to CO2 and its corresponding graph after 30 minutes. ADI lets you keep the settings if you are photographing the same thing over time.
24 This is a qualitative graph of diffusion At 30 minutes, the point at which the CO2appears to have diffused is 10/100.
25 ADI for Quantitative Analysis of Diffusion ADI can be used to quantify diffusion as well.The petri dish in the photo has a known diameter.To quantify diffusion, follow the same steps for opening the photo but choose scale present in image.
30 Draw a diagonalacross the petri dishRecord actuallength
31 Draw line across gelatin Length of gelatin lineZoom in to help with accuracy
32 It looks like the diffusion ends Draw a line to whereIt looks like the diffusion endsLength of diffusion line
33 Future Inquiry Possibilities Using the Digital Camera as a data collection tool, observe how changing: temperature; density of gelatin; or concentration of the solute (CO2)--to mimic increasing CO2 levels--affects the rate of diffusion.