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Expression level ARR3 - + - + - + - + BA 30 120 min 200 150 100 50 0 ARR4 5000 4000 3000 2000 1000 0 - + - + - + - + BA 30 120 min ARR5 5000 4000 3000.

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Presentation on theme: "Expression level ARR3 - + - + - + - + BA 30 120 min 200 150 100 50 0 ARR4 5000 4000 3000 2000 1000 0 - + - + - + - + BA 30 120 min ARR5 5000 4000 3000."— Presentation transcript:

1 Expression level ARR BA min ARR BA min ARR BA min ARR BA min ARR BA min Expression level ARR BA min Expression level ARR BA min ARR BA min ARR BA min Col-0 35S:ARR BA min AHK AHK BA min Expression level Supplementary Figure 1 Analysis of A-type ARRs, AHK1, and AHK4 gene expression in response to cytokinins in wild type and 35S:ARR7. Seven-day-old light-grown seedlings of wild-type and 35S:ARR7 were either mock-treated or treated with 5 M BA for 30 min or 2 h, total RNA was isolated, and followed by the GeneChip experiments. Cytokinin-regulated A-type ARR and AHK genes that exhibit, on average, >2-fold change are presented. The expression levels (y axis) represent differential hybridizations between perfectly matched and mismatched oligodeoxyribonucleotides and average values of two independent biological experiments. Shaded and thick columns indicate wild-type and 35S:ARR7 transgenic plants, respectively

2 EXPA Expression level EXPA BA min BA min EXPA BA min EXPA BA min EXPB BA min EXPA BA min EXPA BA min Expression level EXPA BA min Expression level Supplementary Figure 2 Analysis of expansin gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1

3 LBD BA min LBD BA min Expression level Supplementary Figure 3 Analysis of LBD gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1

4 CKX2CKX3 CKX4 CKX5 CKXa BA min Expression level BA min BA min BA min BA min Expression level Supplementary Figure 4 Analysis of cytokinin oxidase gene expression in response to cytokinins in wild- type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1

5 At1g At4g15680 Expression level At2g BA min At3g Expression level BA min BA min BA min Supplementary Figure 5 Analysis of glutaredoxin gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1


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