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02101620468121418 Retention time (min) Relative intensity Leaf ESI (+) ESI () Supplementary Figure 1 Total ion current chromatogram of lipids from rice.

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Presentation on theme: "02101620468121418 Retention time (min) Relative intensity Leaf ESI (+) ESI () Supplementary Figure 1 Total ion current chromatogram of lipids from rice."— Presentation transcript:

1 02101620468121418 Retention time (min) Relative intensity Leaf ESI (+) ESI () Supplementary Figure 1 Total ion current chromatogram of lipids from rice leaves. Supplementary Figure 1 PC PI PE DGDG PG SQDG GlcCer MGDG SG

2 Supplementary Figure 2. Structural characterization of triterpenoid saponins. (A) Positive MS/MS of tomatine, and (B) avenancin A-1 are displayed. The m/z values of precursor ions are shown in parentheses. Gal, galactose; Glc, glucose; Xyl, xylose; Ara, arabinose; N-MeAnth, N-methylanthranilic acid. A Relative Intensity (%) B 30050060080090010001100700400 m/z 100 75 50 25 0 100 75 50 25 0 m/z 638.404 m/z 932.496 m/z 469.330 m/z 770.439 m/z 451.319 m/z 423.319 Glc ( 162) Glc ( 162) Ara ( 132) N-MeAnth ( 151) (m/z 1094.549) (m/z 1034.535) 30050060080090010001100700400 Glc ( 162) Xyl ( 132) Glc ( 162) Gal ( 162) m/z 578.403 m/z 416.353 m/z 902.515 m/z 740.458 Supplementary Figure 2

3 Supplementary Figure 3 Supplementary Figure 3 TGDG in tgd3 mutant. (A) Positive MS of TGDG in tgd3 mutant (t R 12.5 min). (B) Analysis of 34:6 TGDG in the wild type and tgd3 mutant. The intensity of extracted ion chromatogram of [M+NH 4 ] + of 34:6 TGDG in the tgd3 is set as 100%. The baseline is shifted for convenience. (C) Heat map expression of profiles of molecular-related ions of TGDG in wild type and tgd3 mutant. Each colored bar within a column represents an ion detected by positive LC-MS in the crude lipid extracts of the wild type and tgd3 mutants (biological replicates = 4). Peak picking and alignments were performed using Shimadzu Profiling Solution software (tolerance for m/z variation, 20 mDa; noise level, 2e5; tolerance for t R variation, 0.1 min). Deisotope and deconvolution procedures were omitte. Peak intensities were normalized based on [M+H] + of internal standard. The color of each bar represents the z-scored intensity of the ions of the corresponding lipid species. m/z 1089.678, [M+NH 4 +1] + of 34:6 TGDG m/z 1093.634, [M+Na] + of 34:6 TGDG m/z 1088.671, [M+NH 4 ] + of 34:6 TGDG Col-0tgd3 -2.91.02.9 02101620468121418 Retention time (min) Relative intensity tgd3 WT B 34:6 TGDG A Relative Intensity (%) m/z 100 75 50 25 0 m/z 1088.677, [M+NH 4 ] + of 34:6 TGDG m/z 1109.600, [M+K] + of 34:6 TGDG m/z 1093.628, [M+Na] + of 34:6 TGDG C 900950100010501100115012001250 not detected

4 Supplementary Figure 4. Structural characterization of two types of 34:6 DGDG (A) Negative MS/MS of 34:6 Gal(16) GalDG and (B) 34:6 Gal(16) GalDG are displayed. The m/z values of precursor ions are shown in parentheses m/z A Relative Intensity (%) 100 75 50 25 0 3004006007001000500 2×Gal–2×H 2 O ( 324) 800900 m/z 907.520, [M–H] – m/z 629.312 m/z B Relative Intensity (%) 100 75 50 25 0 3004006007001000500 2×Gal–2×H 2 O ( 324) 800900 m/z 907.521, [M–H] – m/z 629.314 (m/z 953.5, [M+HCO 2 ] – ) Supplementary Figure 4

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