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Supplemental Figure 1 FLAG/Luc/#1 FLAG/Keap1/#1 A Vimentin FLAG/Luc/#1FLAG/Keap1/#1 B.

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Presentation on theme: "Supplemental Figure 1 FLAG/Luc/#1 FLAG/Keap1/#1 A Vimentin FLAG/Luc/#1FLAG/Keap1/#1 B."— Presentation transcript:

1 Supplemental Figure 1 FLAG/Luc/#1 FLAG/Keap1/#1 A Vimentin FLAG/Luc/#1FLAG/Keap1/#1 B

2 A C 0 5,000,000 10,000,000 15,000,000 20,000,000 25,000,000 Nrf2 shRNA/#2 Relative Intensity Control GSH Number x 10 Levels of ROS B Nrf2 shRNA/#2 Control shRNA Supplemental Figure 2 C400 Cell Number ControlNrf2 shRNA

3 Supplemental Figure 3 Control shRNA pAKT AKT GAPDH Cyclin D1 Nrf2 shRNA #2 C-Jun

4 Supplemental Figure 4 Control lentivirus shRNA Lentivirus Nrf2 shRNA clone 3 Lentivirus Nrf2 shRNA clone 6 Cell Number Days Control A549 cells Lentivirus Nrf2 shRNA clones Clone 3 Nrf2 GAPDH B Clone 6

5 Supplemental Figure 5 Control shRNA Nrf2 shRNA

6 5 ng/ml TGF- SB TGF- 0 hr 24 hr 24 hr Supplemental Figure 6 Nrf2 siRNA in pSilencer Control siRNA in pSilencer O hr 24hr A C Control retrovirus shRNA Retrovirus Nrf2 shRNA #2 24 hrs B

7 24 hrs + TGF- 24 hrs TGF- + SIS3 Supplemental Figure 7

8 Supplemental Figure 8 A side media ports Cell loading port B side media ports 6 micron posts 8 micron posts 10 micron posts 12 micron posts Micro-Taur schematic diagram Insert illustrating Post Region (12 micron) Post- containing chambers

9 Nrf2-shRNA #2 Supplemental Figure 9 TGF- 1 pg/ml Control shRNA

10 TGF β R-Smad PP MH1 MH2 R-Smad MH1 MH2 Phosphatases Slug E-Cadherin Smad target genes Nrf2 pR-Smad Smad4 Smad target genes pR-Smad Smad4 R-Smad P PP MH1 MH2 Phosphatases ROS Loss of Nrf2 expression ROS Nrf2 R-Smad P PP MH1 MH2 R-Smad P PP MH1 MH2 Supplemental Figure 10 A B Smad target genes pR-Smad Smad4


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