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Published byKaylee Leblanc Modified over 3 years ago

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Supplementary Figure 1. ER stress-induced secretion of mature IL-1β by THP-1 cells is dependent on the NLRP3 inflammasome. MSU 300 MockR837 15 shRNA :ScrambleNLRP3ASC Tunica 101520MockR837 15101520 MockR837 15101520MockR837 15101520 Tunica Caspase-1 µg/ml SN xT IL-1 pIL-1 pCasp1 ASC NLRP3 CHOP Sup. Fig. 1

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Supplementary Figure 2. ER stress-induced activation of the NLRP3 inflammasome is independent of TLR and RLR signaling. BFA 0.1 MSU 150 µg/ml CB A mockR837 15Tunica 15 µg/ml SN xT shRNA : ScrambleMyD88 mockR837 15Tunica 15 IL-1 pIL-1 pCasp1 MyD88 MSU 150 µg/mlMockThapsi 10 µMTunica 20 µg/ml Trif+/+Trif-/- Tunica 10 µg/mlThapsi 5 µMMSU 150 µg/mlMockThapsi 10 µMTunica 20 µg/mlTunica 10 µg/mlThapsi 5 µM SN xT IL-1 pIL-1 pCasp1 Casp1 pIL-1 pCasp1 CHOP MockThapsi 10 µMTunica 20 µg/ml Mavs+/+Mavs-/- Tunica 10 µg/mlThapsi 5 µMMockThapsi 10 µMTunica 20 µg/mlTunica 10 µg/mlThapsi 5 µM SN xT pIL-1 IL-1 pCasp1 Casp1 pIL-1 pCasp1 CHOP Sup. Fig. 2

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Supplementary Figure 3. IRE1α shutdown in shRNA-expressing THP-1 cells. shIRE1 #1shIRE1 #2shIRE1 #3Empty vector xT IRE1α Tubulin Sup. Fig. 3

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Supplementary Figure 4. TRAF2 and ASK1 are dispensable for ER stress-induced NLRP3 inflammasome activation. B A Mock ScrambleshRNA : MSU 300 µg/mlR837 20 µg/mlTunica 10 µg/mlMockMSU 300 µg/mlR837 20 µg/mlTunica 10 µg/mlMockMSU 300 µg/mlR837 20 µg/mlTunica 10 µg/ml TRAF2 #1TRAF2 #2 xT SN IL-1 pIL-1 TRAF2 MSU 300 µg/mlMockThapsi 5 µMTunica 10 µg/ml Ask1+/+Ask1-/- Tunica 5 µg/mlThapsi 2.5 µMMSU 300 µg/mlMockThapsi 5 µMTunica 10 µg/mlTunica 5 µg/mlThapsi 2.5 µM xT SN pIL-1 pCasp1 Casp1 Sup. Fig. 4

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