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LS174T p-EGFR MEK 1/2 p-MEK 1/2 AKT actina RAS-T RAS-Act pAKT EGFR GEO HCT15 HCT116 SW480 SW620 p-MEK 1/2 actina RAS-Act p-EGFR MEK 1/2 AKT RAS-T pAKT.

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Presentation on theme: "LS174T p-EGFR MEK 1/2 p-MEK 1/2 AKT actina RAS-T RAS-Act pAKT EGFR GEO HCT15 HCT116 SW480 SW620 p-MEK 1/2 actina RAS-Act p-EGFR MEK 1/2 AKT RAS-T pAKT."— Presentation transcript:

1 LS174T p-EGFR MEK 1/2 p-MEK 1/2 AKT actina RAS-T RAS-Act pAKT EGFR GEO HCT15 HCT116 SW480 SW620 p-MEK 1/2 actina RAS-Act p-EGFR MEK 1/2 AKT RAS-T pAKT GLC82 A549 Calu3 H460 H1299 EGFR Fig. S1 A B

2 GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE p.G12Dc.35G>ALS174T p.G12Ac.35G>CGEO p.G12Vc.35G>TSW480;SW620 p.G13Dc.38G>AHCT116; HCT15 p.G13Ac.38G>C p.G13Vc.38G>T p.G13Gc.39C>G p.G13Gc.39C>A p.G13Gc.39C>T p.G12Sc.34G>AA549 p.G12Rc.34G>C p.G12Cc.34G>T p.A59Tc.175G>A p.Q61Kc.181C>A p.Q61Ec.181C>T p.Q61Ec.181C>G p.Q61Pc.182A>C KRAS Table S.1A Mutation Status of Cell Lines Used in This Study

3 GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE p.Q61Rc.182A>G p.Q61Lc.182A>T p.Q61Hc.183A>CH460 p.Q61H c.182A>T KRAS

4 GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE p.Q61Pc.182A>C p.Q61Lc.182A>T p.Q61Rc.182A>G p.Q61Hc.183A>C p.Q61Qc.183A>G p.Q61Hc.183A>T p.G61Kc.181C>AH1299 p.G61Ec.181C>G p.G13Sc.37G>A p.G13Rc.37G>C p.G13Cc.37G>T p.G12Rc.34G>C p.G12Sc.34G>A NRAS Table S.1B Mutation Status of Cell Lines Used in This Study

5 GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE p.K601Ec.1801A>G p.V600Ec.1799T>A p.D594Gc.1781A>G BRAF Table S.1C Mutation Status of Cell Lines Used in This Study

6 GENEAMINO ACID CHANGE NUCLEOTIDE MUTATION EXONCELL LINE p.H1047Rc.3140A>Gexon 20HCT116; GLC82; LS174T p.H1047Lc.3140A>Texon 20 p.P539Rc.1616C>Gexon 9 p.Q546Kc.1636C>Aexon 9A549 p.Q546Ec.1636C>Gexon 9 p.E81Kc.241G>Aexon 1 p.R88Qc.263G>Aexon 1 p.G106Vc.317G>Texon 1 p.N345Kc.1035T>Aexon 4 p.R93Wc.277C>Texon 1 p.S158Lc.473C>Texon 2 p.H160Nc.478C>Aexon 2 p.R38Hc.113G>Aexon 1 p.E542Kc.1624G>Aexon 9 p.E542Qc.1624G>Cexon 9 p.E545Kc.1633G>Aexon 9H460;HCT15 p.E545Qc.1633G>Cexon 9 PIK3CA Table S.1D Mutation Status of Cell Lines Used in This Study

7 GENEAMINO ACID CHANGE NUCLEOTIDE MUTATION EXONCELL LINE p.G118Dc.353G>Aexon 2 p.G12Dc.35G>Aexon 1 p.H1047Yc.3139C>T exon 20 p.C420Rc.1258T>Cexon 7 p.H701Pc.2102A>C exon 13 p.K184Ec.550A>Cexon 2 p.C901Fc.2702G>Texon 18 p.M1004Ic.3012G>Texon 20 p.G1007Rc.3019G>Cexon 20 p.G1049Rc.3145G>Cexon 20 p.R108H c.323G>A exon 1 PIK3CA

8 GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE p.R248Wc.742C>T p.Y220Cc.659A>G P53 GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE p.F241Sc.722T>C p.Y65Cc.194A>G p.E150Qc.448G>C p.K62Rc.185A>G p.K267fs*9c.800delA PTEN GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE p.R154Wc.460C>T p.E221c.661G>T p.S251Nc.752G>A p.I295fs*23c.882_882delG MEK 1/2 Table S.1E Mutation Status of Cell Lines Used in This Study

9 GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE AKTp.A377Vc.1130C>T GENEAMINO ACID CHANGENUCLEOTIDE MUTATIONCELL LINE EGFRp.E746_A570c.2236_2250del15 Table S.1F Mutation Status of Cell Lines Used in This Study

10 KRAS PI3Kca BRAF NRAS NOYES RE25 SE31 Χ-testp = RESE No mut12 G1223 G1311 Χ-testp = NOYES RE70 SE31 Χ-testp = 1 NOYES RE14 SE24 Χ-testp = KRAS mutation NRAS mutation PIK3Ca mutation KRAS (G12and G13) mutation Fig. S

11 Fig. S7 H460A549LS174THCT15GLC82CALU3HCT116H1299SW620SW480GEOH460A549LS174THCT15GLC82CALU3HCT116H1299SW620SW480GEO Fig. S3

12 αsαs β γ GPCR RTK Ras Raf-1 MEK1/2 MAPK ATP cAMP PKA R PKA C AKAP13 RA signaling Cell proliferation Cell differentiation Cell survival Cell proliferatiom TAF3 RXRANR1H2 NCOA3 Fig. S4 P P P P Grb2 SOS Shc ADCY7


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