1 - Define the term plasmid and explain its significance for bacteria and recombinant technology. Go to http://www.microbiologyprocedure.com/microbial- genetics/gene-cloning-procedure.htm orhttp://www.microbiologyprocedure.com/microbial- genetics/gene-cloning-procedure.htm http://faculty.plattsburgh.edu/donald.slish/Transforma tion.html Explain what a plasmid is and why it is so vital for recombinant technology. Extra – what other vectors are there besides plasmids?
2 - Describe how genomic and plasmid DNA can be isolated from cells. Go to http://www.iupui.edu/~wellsctr/MMIA/isolati ng_dna/dna_isolation_rev.swf http://www.iupui.edu/~wellsctr/MMIA/isolati ng_dna/dna_isolation_rev.swf Watch the animation. In a paragraph or two, in your own words, describe the steps and their reasons in the DNA isolation process.
3 - Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. Look at the picture above, in your own words, explain why sticky ends are necessary when splicing together a recombinant DNA molecule.
4 - Explain each of the steps taken to isolate a gene of interest and insert it into bacterial DNA to produce the desired protein. Watch the tutorial http://www.sumanasinc.com/webcontent/anima tions/content/plasmidcloning.html http://www.sumanasinc.com/webcontent/anima tions/content/plasmidcloning.html Create step by step instructions, assume you are creating these instructions for a lab manual, explaining what must be done in each step and why, to isolate a specific gene ( we are assuming it is the gene to make you orange and blue!) and insert it into an E.Coli cell.
5 - Identify ways to determine if genetic transformation has been successful. three Using our notes (PPT), give three protocols for markers of successful transformation in an E. coli. How can we tell our transformation occurred?
8 - Relate bacterial transformation to real life applications used by society. Speak to other students in the class, each person did a brochure on a real life application of bacterial transformation. Give one example of real life applications in….. 1.Agriculture – plants 2.Agriculture – animals 3.Medicine – production of medicines 4.Medicine – gene therapy 5.Transgenic organisms for research purposes