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Published byQuentin Dennis
Modified over 7 years ago
POLYMERASE CHAIN REACTION AMPLIFYING DNA What do you need to replicate DNA? http://www.youtube.com/watch?v=uD umZT5z5R8
http://www.dnalc.org/view/15475-The-cycles-of-the- polymerase-chain-reaction-PCR-3D-animation-with-no- audio.html
WHICH DNA SEQUENCE IS AMPLIFIED?
TANDEM REPEATS (VNTR)
TANDEM REPEAT GENES 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’
DETERMINING YOUR ALLELES CREATING DNA FINGERPRINT USING GEL ELECTROPHORESIS
SAMPLE GEL Ladder Suspect 1 Suspect 2 Crime Scene 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’
HOW UNIQUE ARE YOU?
RFLP RESTRICTION FRAGMENT LENGTH POLYMORPHISM
EXAMPLE USE TTCC RESTRICTION ENZYME DNA SAMPLE ONE: ACTGTTCCACACTTCCGCACATCTTAATTCC DNA SAMLE TWO: GTTCCATGCCGAGTACGTTCCATGCTA
I AM YOUR FATHER….WELL MAYBE
DNA Fingerprinting and Forensic Analysis Chapter 8.
Explain how crime scene evidence is
C rime S cene I nvestigation. Who Ate The Cheese? Oh no! Someone ate the Queen ’ s prize Limburger cheese! Saliva samples were taken from the teeth marks.
The Biology Behind DNA Fingerprinting Mark Bailey.
Molecular Biology of Genes Chapters DNA Technology (not in your book)
What are the three steps in PCR?. Denaturation Hybridization of Primer DNA replication.
DNA basics DNA is a molecule located in the nucleus of a cell Every cell in an organism contains the same DNA Characteristics of DNA varies between individuals.
Biotechnology. Comparative genomics also has been used to identify recently mobilized transposons in genetically diverse humans. For example, over 600.
explain how crime scene evidence is
1 Chapter 7 Chapter 7 DNA Fingerprinting Learning Goals: o Explain how crime scene evidence is collected and processed to obtain DNA o Describe how radioactive.
Aim: How do scientists identify people using DNA Fingerprinting?
The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints
DNA Fingerprinting or DNA Profiling
DNA Fingerprinting. We share 99.9% of our DNA with each other. That means the 0.1% of our DNA makes us unique. But that is still is over 3,000,000 differences!
DNA Profiling in Forensic Science. Introduction DNA Profiling is the analysis of DNA samples to determine if they came from the same individual. Since.
Class Notes 1: DNA Manipulation. I. DNA manipulation A. During recent years, scientists have developed a technique to manipulate DNA, enabling them to.
Manipulation of DNA. Restriction enzymes are used to cut DNA into smaller fragments. Different restriction enzymes recognize and cut different DNA sequences.
15.3 Gene Technologies in Detail Mrs. Stipanovich.
DNA FINGERPRINTING. 1.What do you think DNA fingerprinting is? 2. What do you think DNA fingerprinting can be used for?
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