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Aim: How do scientists identify people using DNA Fingerprinting?

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Presentation on theme: "Aim: How do scientists identify people using DNA Fingerprinting?"— Presentation transcript:

1 Aim: How do scientists identify people using DNA Fingerprinting?

2 LO: SWBAT explain how scientists identify people using DNA fingerprinting
DN: Why do we fingerprint people? What is it useful for? HW: read pp , page 339 #18-19 Fri: Review Book- Respiration Quarterly- Next Wed/Thurs

3 Why do we Fingerprint? So we can identify people

4 Why is DNA used to identify people?
People have different DNA base sequences, therefore each individual will have a different DNA fingerprint.

5 Genetic Markers: ATTGCCTAGG - Individual 1 ATTGCCATGG - Individual 2
particular stretches of ____ that are _________ among individuals. DNA variable Our __________ regions are highly _________ from one individual to another and serve as good ________________ to be used for DNA fingerprinting. noncoding variable genetic markers

6 How do scientists make a DNA Fingerprint?
Investigators need to find some ____ of the perpetrator. DNA Where can the investigators find the DNA? Blood Hair Nails Skin

7 Only a small amount of DNA is needed!
Polymerase Chain Reaction: PCR The sample of DNA is amplified (___________) multiplied

8 PCR Animation

9 – + Gel Electrophoresis
Amplified DNA is cut with specific __________________. 2) DNA segments are loaded into wells in a porous ____. 3) Electric current is passed through the gel making one side of the gel __ charged and one side __ charged. restriction enzymes gel +


11 Gel Electrophoresis DNA is ___________ charged & moves through the gel to the _________ side. 5) Smaller segments move _______ & ________ than the larger segments. negatively positive faster farther


13 Gel Electrophoresis 6) The gel is then ________ so we can see the DNA ________ patterns. stained banding Result: The smaller segments of DNA are found closer to the _________ end than the larger segments. positive


15 **Separates DNA segments
according to _____. SIZE

16 - + Gel Electrophoresis __ __ __ __ __ __ __ __ __ __
DNA Suspect A Suspect B Suspect C from CS __ __ __ __ __ __ __ __ __ __ +

17 DNA Fingerprint Questions:
What is the charge of DNA? Where would we find the smallest DNA fragments, closer to the + end or the – end? Why do we get different banding patterns for each individual? 4) Which suspect was at the crime scene?

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