So we can just keep splitting it open and adding bases and make millions and millions of copies. This is amplification. Technically, this is not a science term either. Amplify: Root words Amplus, large + facere, to make* *Websters New World Compact School and Office Dictionary, at 15, (1989)
Holt Science and Technology, Life Science, p. 137 The loci we care about are tagged with a flourescent dye...
AMEL D3S1358 TH01 TPOX D2S1338 D19S433 FGA D21S11 D18S51 CSF1PO D16S539 D7S820 D13S31 7 D5S818 VWA D8S1179 See the funny looking ruler at the top? That measures the number of base pairs. X is about 106 base pairs long. The 24 at D2 is 344 base pairs long. AmpFlSTR ® Identifiler (Applied Biosystems) Slide courtesy of John Butler Why do we care about how long these things are? The length is one of the things that tells us what these things are.
AMEL D3S1358 TH01 TPOX D2S1338 D19S433 FGA D21S11 D18S51 CSF1PO D16S539 D7S820 D13S31 7 D5S818 VWA D8S1179 The importance of color... AmpFlSTR ® Identifiler (Applied Biosystems) Slide courtesy of John Butler
Quantity of DNA The amount of DNA is measured on the right.
WHAT DOES THIS MEAN?? The DNA profile found at the crime scene Matches the DNA profile said to be from the defendant...
Lets compare DNA profiles: Stand up! (Humor me, would ya?) Match is with female With white skin And blue eyes And red hair Who was raised catholic By an Irish mother And has one child
Whos in the mix? Its time to stand up!! Match is with female or male With white skin or olive skin And blue eyes or brown eyes And red hair or black hair Who was raised catholic or free spirit By an Irish mother or an Italian one And has one daughter or one son
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