1. Magnetic Material Engineering

2. Chapter 6: Applications in Medical and Biology Magnetic Material Engineering

3. Chapter 6: Applications in Medical and Biology Magnetic nanoparticles

4. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic nanoparticles

5. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic nanoparticles

6. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic nanoparticles Materials: Fe, Co, Ni, Gd Spins of unfilled d-bands spontaneously align parallel inside a domain below a critical temperature T C (Curie) Laws: B = H +4  H M =  H  : Susceptibility

7. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic nanoparticles Hard Magnets: H C and M rs have high values

8. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Superparamagnetism - a size effect

9. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Superparamagnetism - a size effect Superparamagnetism: high saturation magnetisation M S no remanence M R = 0

10. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Superparamagnetism The Néel relaxation in the absence of magnetic field Normally, any ferromagnetic or ferrimagnetic material undergoes a transition to a paramagnetic state above its Curie temperature. Superparamagnetism is different from this standard transition since it occurs below the Curie temperature of the material. Superparamagnetism occurs in nanoparticles which are single domain. This is possible when their diameter is below 3–50 nm, depending on the materials. In this condition, it is considered that the magnetization of the nanoparticles is a single giant magnetic moment, sum of all the individual magnetic moments carried by the atoms of the nanoparticle.

11. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Superparamagnetism Magnetic anisotropy is the direction dependence of a material's magnetic properties. In the absence of an applied magnetic field, a magnetically isotropic material has no preferential direction for its magnetic moment while a magnetically anisotropic material will align its moment with one of the easy axes. Magnetic anisotropy is a prerequisite for hysteresis in ferromagnets: without it, a ferromagnet is superparamagnetic.

12. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic anisotropy Are the magnetic properties same in all directions?  No It depends on the crystallographic direction in which the magnetic dipoles are aligned – Crystal anisotropy (Spin Orbit Coupling) – Shape anisotropy – Stress anisotropy – Externally induced anisotropy – Exchange anisotropy E = KVsin 2 θ (simplest form) – K the effective uniaxial anisotropy energy per unit volume – V particle volume – θ angle between moments and easy axis

13. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic anisotropy

14. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Superparamagnetism Because of the nanoparticle’s magnetic anisotropy, the magnetic moment has usually only two stable orientations antiparallel to each other, separated by an energy barrier. The stable orientations define the nanoparticle’s so called “easy axis”. At finite temperature, there is a finite probability for the magnetization to flip and reverse its direction. The mean time between two flips is called the Néel relaxation time τ N and is given by the following Néel-Arrhenius equation:

15. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Superparamagnetism  N is thus the average length of time that it takes for the nanoparticle’s magnetization to randomly flip as a result of thermal fluctuations.  0 is a length of time, characteristic of the material; its typical value is 10 −9 –10 −10 second. K is the nanoparticle’s magnetic anisotropy energy density and V its volume. KV is therefore the energy barrier associated with the magnetization moving from its initial easy axis direction, through a “hard plane”, to the other easy axis direction. k B is the Boltzmann constant andT is the temperature.

16. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Superparamagnetism - a size effect

17. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Superparamagnetism - a size effect

18. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic Nanoparticles Cancer Treatments MRI Drug Delivery Manipulation of Cell membranes Cell Separation Cell Separation

19. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Nanomagnetic Particle

20. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology

21. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology

22. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic Cell Separation / Cell Labeling Diagram of Immunomagnetically labeled Cell

23. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic Cell Separation / Cell Labeling

24. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Capture of bacteria by using Vancomycin-conjugated magnetic nanoparticle Control experiment

25. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic Drug Delivery

26. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology MRI The principles of MRI rely on the spinning of specific nuclei present in biological tissues. In nuclei that have an even mass number, i.e. # protons = # neutrons, half spin in one direction and half spin in the other. Nucleus has no net spin. However, in nucleus with odd mass #, spin directions are not equal and opposite, so the nucleus has net spin or angular momentum. These are know as MR active nuclei.

27. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology MRI MR active nuclei are characterized by their tendency to align their axis of rotation to an applied magnetic field. This occurs because they have angular momentum or spin and, as they contain positively charged protons, they posses electrical charge. The laws of electromagnetic induction refer to three individual forces – motion, magnetism and charge – and state that if two of these are present, then the third is automatically induced. MR active nuclei that have a net charge and are spinning (motion), automatically acquire a magnetic moment and can align with external magnetic field. The strength of the total magnetic moment is specific to every nucleus and determines the sensitivity to magnetic resonance.

28. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology MRI - The hydrogen nucleus The hydrogen nucleus is the MR active nucleus used in clinical MRI. It contains a single proton (atomic and mass number 1). It is used because it is most abundant in the human body and its solitary proton gives it a relatively large magnetic moment. Both of these characteristics enable utilization of the maximum amount of available magnetization in the body.

29. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology The hydrogen nucleus as a magnet The laws of electromagnetism state that a magnetic field is created when a charged particle moves. The hydrogen atom contains one positively charged proton that spins. Therefore it has a magnetic field induced around it, and acts as a small magnet. It has a north and south pole. Each of which is represented by a magnetic moment.

30. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Alignment Quantum theory describes properties of electromagnetic radiation in terms of discrete quantities called quanta. Applying quantum theory to MRI, hydrogen nuclei posses energy in two discrete quantities term low and high. Low energy align their magnetic moments parallel to external field (spin- up). High energy align anti-parallel (spin-down).

31. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology The MR signal When a patient is placed within and MR scanner, the protons in the patients tissues (primarily protons contained in water molecules) align themselves along the direction of the magnetic field. A radiofrequency electromagnetic pulse is then applied, which deflects the protons off their axis along the magnetic field. As the protons realign themselves with the magnetic field, a signal is produced. This signal is detected by an antenna, and with the help of computer analysis, is converted into an image.

32. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Image formation The process by which the protons realign themselves with the magnetic field is referred to as relaxation. Different tissues undergo different rates of relaxation, and these differences create the contrast between different structures, and the contrast between normal and abnormal tissue, seen on MRI scans.

33. Magnetic Material Engineering Chapter 6: Applications in Medical and Biology MRI of a Female Rat BeforeAfter