1. Tissue Engineering Lecture 13, 3/26/15 Paper Review 3D gels for ischemia

2. What is the motivation of this study? Much of current cell therapy relies on dosing cells at the site – many of which are lost during delivery. One mechanism to improve cell therapy is to encapsulate cells into a biomaterial – keeping the cells alive during the harsh delivery. The authors propose to deliver adipose(fat)- derived mesenchymal stem cells in very small injectable gels to protect them during delivery.

3. Show supplementary video 1

4. Figure 1a-e: Jemima Lamothe

5. Figure 1f-i: Jonathan Hummel

6. Figure 2a-c: Michael Mulroy

7. Figure 2d: Ezra Aurian-Blajeni

8. Figure 3: Sarah Johnson

9. Figure 4a-b: Kyle Pariseau

10. Figure 4c-d: Ronen Zeidel

11. Figure 5: Matt Tiller

12. Show supplementary movies 2-4

13. Figure 6: Christine Davis

14. Conclusions, Perspectives By encapsulating cells in 3D microniches, they could use a dose more than 10 times lower than previous cell based therapies. Accumulation of ECM and paracrine signaling in the niches likely provided survival signals to cells. Priming was a key step to improving MSC differentiation prior to delivery.