Lock & Key Model One enzyme for every substrate for every substrate unique fit Enzyme Substrate
Enzymes are temperature dependent…... Most work best at body temperature 37oC Denatured at high temperatures Inactive at low temperature Temperature o C
Effect of pH on enzyme activity Optimum pH : the pH value at which the enzyme has the highest activity (fastest rate of reaction) Different enzymes may have different optimum pH’s pH of substrate higher or lower than optimum pH, it can denature the enzyme making it ineffective.
Enzyme Lab Enzyme: catalase Source: liver, potato, apple etc Substrate: H 2 O 2 Hydrogen peroxide is toxic in cells 2H 2 O 2 +catalase 2 H 2 O + O 2 2 H 2 O + O 2 Enzymes end in ‘ase’