1. Lecture 4 Announcements 1.Email me if you did NOT receive a message from me last Friday. 2.BlackBoard kinda works but we will stay with our own site for now: www.uky.edu/~gcbrown/ent561 www.uky.edu/~gcbrown/ent561 3.Will try to get the lecture notes on sooner. 4.No quiz on Thursday 5.Online Reading: http://www.crid.or.cr/digitalizacion/pdf/eng /doc14606/doc14606-10.pdf

2. Epidemic Components 1. The etiologic agent = microorganism that can cause disease. –Pathogenicity = its ability to cause disease; – Virulence = the severity of infection, expressed as: morbidity = incidence of disease mortality = death rate of the infection. 2. The method of transmission = means by which the agent goes from the “source” to the host. We are only concerned with vectored pathogens here. 3. The host – Depends on 3 things: –Accessibility –Susceptibility –Transmissibility

3. Most studies of arthropod-vectored disease study: Time Trends – Four kinds –The secular trend - occurrence of disease over years –The periodic trend – temporary change in a secular trend, –The seasonal trend - seasonal changes in disease occurrence –The epidemic occurrence of disease. Place Considerations –where the individual was when disease occurred; –where the individual was when he or she became infected; –where the vector became infected with the etiologic agent. Infected Person –Individual characteristics: age, sex, occupation, personal habits, underlying disease, immunization history, etc. –Social characteristics: socioeconomic status, geographical distribution, public works & policies, disease rates in other group members

4. Example: Rocky Mountain Spotted Fever Etiological Agent – Rickettsia rickettsii Vector – Dog & wood ticks

5. Example: Rocky Mountain Spotted Fever Secular trend of the disease www.cdc.gov/ncidod/dvrd/rmsf/Epidemiology.htm Periodic Trend

6. Example: Rocky Mountain Spotted Fever Seasonal Trend

7. Example: Rocky Mountain Spotted Fever Epidemic Time Period

8. Example: Rocky Mountain Spotted Fever Geography Age

9. Parasite types in Medical Entomology Micro vs macroparasites – Micro are microorganisms (viruses, bacteria, protozoa, etc.). Macro are arthropods. Ectoparasites vs endoparasites – –Ecto – parasitize from outside the host, infestation normally remains topical and peripheral –Endoparasites – parasitize from inside the host, Microparasites can produce systemic infestation. Disease is normally systemic. Facultative vs. obligate – –Obligate are totally dependent on host. –Facultative have parasitic and free living forms.

10. Vectors and bloodfeeding Most vectors are blood-feeders: –Normally the pathogen does not benefit the vector, detrimental effects sometimes occur. –The pathogen receives many benefits (acqusition, transport, re-insertion). –Principal problems for the pathogen from this system: Must overcome ingestion by the vector (next slide) Must be able to infect a new host from a small inoculum (slide following) Totally dependent on the vector-host population dynamics and interaction.

11. 1.Pathogen ingested 2.Pathogen may multiply here (plague bacteria, Leishmania) 3.Passage through gut wall (filarial worms, Borellia) or multiplication in epithelial layer (rickettsiae, arboviruses), or cysts (malaria) 4.Transport by hemolymph to tissues (some arboviruses multiply here too) 5.Tissue concentration in (a) salivary glands (b) reproductive system

12. Infection 6.Contaminated mouth (fluids, mouthparts, escape through body wall) 7.Infected feces 8.Infective fluids (e.g. coaxial gland fluids in ticks) 9.Crushing/ingestion of vector

13. Definitive vs. Intermediate Hosts Definitive Host in which gametocycte union occurs for the parasite. AKA – Primary host Intermediate Host in which asexual reproduction occurs for the parasite. AKA – Secondary host; Alternate host

14. Example: Lymphatic Filariasis Human – Definitive Host, Mosquito the intermediate host

15. Opposite Example: Malaria Mosquito definitive host, human is the intermediate host

16. More about hosts accidental host one that accidentally harbors an organism that is not ordinarily parasitic in the particular species. amplifying host one that increases parasite numbers &/or no. infectious vectors. Typically short-lived infections. dead-end host the disease cannot be transmitted from the infected host to another animal. predilection host the host preferred by a parasite. reservoir host an animal (or species) that is infected by a parasite, and which serves as a source of infection for humans or another species. transfer host, transport host one that is used until the appropriate definitive host is reached, but is not necessary to complete the life cycle of the parasite.

17. About Vectors – Host Selection ‘phagic’ – feeds successfully; ‘philic’ – attracted to. Anthropophagic – feed successfully on humans; Anthropophilic – attacks humans Zoophagic – feed on vertebrates other than humans. Two big subsets –Mammalophagic –Ornithophagic

18. Modes of Transmission Two main modes: –VERTICAL – vector to vector in subsequent life stages or generations –HORIZONTAL – passage of parasites between vectors and hosts

19. Vertical Transmission Subtypes Transstadial – between subsequent life stages Transgeneration – between subsequent generations (several methods) Venereal – passage of parasites occurs between male and female during mating. Rare in vectors

20. Horizontal vs Vertical Transmission

21. Compare Figs. 3.3 & 3.2 in your text 1.Which are the dead end hosts? 2.Where are the bridge vectors? 3.Where is the amplification cycle? 4.Where is the enzootic transmission cycle?

22. Interseasonal Maintenance Most seasonal disease from arthropod-vectored pathogens in temperate zones require interseasonal maint. Principal mechanisms: –Continued transmission – no seasonality, tropical diseases, disease of parasites that continuously live with hosts –Infected Vectors – most encephalitis viruses –Infected hosts – Reservoirs go dormant, parasite has a dormant stage in host. –No interseasonal maintenance – occasional local extinction Migratory hosts Weather fronts

23. Principles of Disease Vector Management Surveillance –Vector, disease prevalence, pathogen burden Prevention –Personal –Public Control –Vector-based –Pathogen-based (pharmacologic)

24. Surveillance Very good general section on this subject in your text: pp: 29 – 32

25. Prevention Personal –Exclusion agents (clothing, screening, bed nets, sealing cracks/crevices, etc.) –Chemical agents (repellents) –Private source controls –Sanitation & Personal Hygiene –Source Avoidance Public –Public service announcements/education –Vector breeding site controls/sanitation –Disaster Management Planning READ: http://www.crid.or.cr/digitalizacion/pdf/eng/doc14606/doc14606- 10.pdf

26. A Key Concept in DV Management is Risk Most DV Management is Govt. Funded They fund efforts in response to perceived risk Risk = Cost of a Bad Outcome TIMES Probability of that Outcome Occurring

27. The main problem is there are multiple risks Multiple “Bad Outcomes” including –Economic –Environmental –Social –Political Each outcome has it’s own cost and probability Major Complication: Disease risk is inversely correlated with vector control.