2. Methodology and scientific achievements of 2003, planning for 2004 Participant 4/Rennenberg Subcontractor 2/Sandermann Freiburg meets Kranzberg forest Experiences of 2003

3. Field campaigns 2003 June July-August September leaves apoplastic solution xylem/phloem roots soil soil water rain water wood

4. Contribution of work Participant 4/RennenbergSubcontractor 2/Sandermann WP1WP2 isotopes (Kris) starch (Kris) sugar (Maria) ascorbate (Kris) glutathione (Maria) salicylic acid (Sascha) gene level (Sascha) ACC (GSF) macro arrays (2004) histochem. analyses (2004 )

5. Links to the other work groups Participant 4 WP1 Participant 4 WP2 Subcontractor2 WP2 Antioxidants/ injury amplification reactions Participant 8 WP2 antioxidants Participant 5 WP2 Participant 6 WP2 Fine-root functionality Participant 2 WP1 Rubisco/ PEPC-activity starch Participant1 Participant7 WP1 Transpiration gH 2 O Participant 3 Subcontractor 1

6. AWF extracted in the laboratory after sampling, N 2 ascorbate, redox state leaves N2N2 ascorbate, glutathione, redox state, salicylic acid, ACC, gene expression δ 13 C; Δ 18 O; δ 15 N, total C/N; starch, sugar xylem Scholander bomb, N 2 glutathione, ascorbate, redox state δ 13 C; Δ 18 O, δ 15 N, total C/N, sugar phloem exudated from bark, N 2 glutathione, ascorbate, redox state δ 13 C; Δ 18 O, δ 15 N, total C/N, starch, sugar wood collected from single twigs ascorbate, redox state δ 13 C; Δ 18 O, δ 15 N, total C/N, starch roots digged out, washed,N 2 ascorbate, redox state, gene expression δ 13 C; Δ 18 O, δ 15 N, total C/N, starch, sugar rainwater collected from a rain barrel, N 2 δ 13 C; Δ 18 O, δ 15 N, total C/N soil water will be provided by δ 13 C; Δ 18 O, δ 15 N, total C/N soil was collected in different height levels (0,5,10,15,20,25 cm), dried δ 13 C, δ 15 N, total C/N

7. Objectives (Kris) δ 13 C: assessment of relative activity changes between C-fixing enzymes (Rubisco/PEPC) δ 13 C is dependent from the CO 2 -concentration in the gaseous phase of the apoplast. The CO 2 -concentration is dependent from assimilation and gH 2 O. Δ 18 O: stomatal conductance WUE: assimilation/transpiration WUE intr : assimilation/gH 2 O 14 N/ 15 N: information about translocation of sugars. total C/N: C/N changes of the whole plant : seasonal/annual time scale starch: carbon fixation through carbohydrates/transport ascorbate: antioxidant status in the whole tree, direct reaction with ROS in the apoplastic solution (hear also Karin)

8. Few scientific achievements......

9. Objectives (Maria) Total sugar in: Leaves Fine roots Phloem Xylem GSH GSSG Redox status of glutathione in: Leaves Fine roots Phloem Xylem

10. Role of Glutathione Glutathione -tripeptide -formed in the leaves -multiple function Effects: activity and expression of genes pool and transport form of reduced S takes part in regulation/assimilation S important antioxidative substance defense against pathogens, heavy metals, etc.

11. SOME EFFECTS OF OXIDATIVE STRESS ON THE PHYSIOLOGY OF PLANTS: Degradation of: Proteins Amino acids Lipids Products of photosynthesis decrease Respiration increases Redox status of GSH and Ascorbate and other antioxidants change Effect on allocation

12. Glucose in leaves

13. Glutathione

14. Conclusions so far -Light decrease of sugar -Increase of GSSG