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From man to mouse … Human PET microPET mouse rat infant monkey human.

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Presentation on theme: "From man to mouse … Human PET microPET mouse rat infant monkey human."— Presentation transcript:

1 From man to mouse … Human PET microPET mouse rat infant monkey human

2 .. and back again

3 Ultrasound PET/SPECT MRI CT Molecular(?) Imaging Modalities Optical(Bioluminescence,fluorescence) Structure Tissue Density, Z A A A A Topography H Concentration Radiotracer M M M ~1-2 mm < mole = quantitative -galactocidase -galactocidase 0.1 µmole H / µmole 31 P ~10 3 cells quantitative quantitative F F F Doppler BOLD, DCE µm 0.1 mm µm to mm

4 Mechanic Collimation ba Pinhole ba Coded Aperture Better Resolution Little Field of View Low Efficiency L d t Resolution & Efficiency inversely related Better Resolution High Efficiency Complicated Reconstruction ba Parallel Holes

5 Normal Myocardial Perfusion at stress and rest Single Vessel Coronary Artery Disease at stress and rest SPECT image exhibits full perfusion (bright, donut shape) both at rest and under stress SPECT image shows limited perfusion (horseshoe shape rather than donut) especially under stress. Cardiac SPECT Images

6 Geant 4 simulation - pixellated CsI(Tl) ( mm pitch) - LaBr3 continuous (3 mm thick, different surface(s) treatment(diffusive vs absorptive) - 6 x 6 mm 2, 3 x 3 mm 2, 1.5 x 1.5 mm 2 (PMT anode pixel size) 1000 counts/view/resol.elem. 1 plaque = 10 mCi,10 resol.elem. aorta: ~ 2 mm diameter plaque size: 0.5 x 1 x 4 mm 3 system sensitivity: ~ 10 cps/ Ci detector area: 100 x 100 mm 2 spatial resolution: ~ 500 m Trying to Image apoptosis by proper tracer (e.g. 99m TcINIC-Annexin-V) Performances not good enough for imaging biological process in vivo in small animals (mice) ManRatMouse Body weight~70 kg~200 g~20 g Brain~105 mm~10 mm~6 mm Heart~300 g~1 g~0.1 g Aorthic cannula~ 30 mm1.5 – 2.2 mm mm (0.5 mm) manrat

7 Preliminary pinhole SPECT reconstruction results from the CsI detector Images are displayed as MIP (maximum-intensity-re-projections) animations 2 point sources APOE mouse (kidneys shown) Flood image Sample projection image Fig. 28 APOE mice

8 Caratteristiche insufficienti per limaging di processi biologici in piccoli animali (topi). E necessario migliorare la risoluzione spaziale e lefficienza ManRatMouse Body weight~70 kg~200 g~20 g Brain (cortex apex- temporal lobe) ~105 mm~10 mm~6 mm Heart ~300 g~1 g~0.1 g Aorthic cannula ( )~ 30 mm1.5 – 2.2 mm mm (0.5 mm) Human Rat 2 mm FWHM (8 mm 3 ) 1 mm FWHM ( 1 mm 3 ) Required spatial resolution: 6 mm FWHM (200 mm 3 ) Small size detectors (high pixellization) Individual detectors or perfect coding

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