1. Microscopes Magnification, Field of View and Estimating Size

2. Eyepiece or Ocular Lens Coarse Focus Knob Fine Focus Knob Arm Stage Clips Base Revolving Nosepiece High Power Objective Lens (40X) Lower Power Objective Lens (10X) Stage Diaphragm Light / Mirror Body Tube

3. Magnification Total magnification is how large the specimen appears under the microscope Total magnification = Eyepiece (ocular) x objective lens Objective Lens Selected Magnification of Objective Lens Magnification of Eyepiece (ocular) Total Magnification Scanning Low Power High Power 4X 10X 40X 10X 40X 100X 400X

4. How much more powerful is high power compared to the low power in terms of total magnification? How much more powerful is high power compared to the scanning in terms of total magnification?

5. Field of View The field of view is the white circle you see through the microscope As you increase magnification the field of view decreases

6. Estimating Size Under a Microscope The field of view will have a different diameter depending on which objective you are using Scanning is usually a few millimeters in diameter, low and high objective are smaller 1mm = 1000 micrometers (μm) or microns