1. Kingdom of Saudi Arabia King Saud University College of Applied Medical Sciences Biomedical Technology Department

2. By: Badr AlShamary & Mohannad Abuljadayel Supervised By: Mr. Mohammed Nisar

3. Polymerase Chain Reaction molecular biological method for amplifying DNA without using a living organism. PCR is commonly used in: Identification of genetic fingerprints. Diagnosis of infectious diseases. Diagnosis of virus diseases.

4. A T G CA TC G The four bases found in DNA are Adenine (A), Cytosine (C), Guanine (G) and Thymine (T).

6. Thermocycler

7. Temperature Graph

8. Sample Contents DNA original Sample Primers DNA Polymerase Buffer Solution Nucleotides(A,T,C and G) Primers DNA Polymerase Buffer Solution Nucleotides

9. PCR Process Step1: Denaturation

10. PCR Process Step2: Annealing

11. PCR Process Step3: Extension

12. PCR Process Doubling DNA each Cycle (Cycle:2)

13. PCR Process (Cycle: 3) Number of copies= 3 2 = 8

14. Virus Diagnosis By PCR Human DNA Virus DNA

15. Human DNA Virus Diagnosis By PCR Virus DNA

16. Human DNA Virus Diagnosis By PCR Virus DNA

17. Thank You