1. 1 15.08.2015 22:56 EasyPreparation without Barcode Reader

2. EasyPreparation without Barcode Reader 2 15.08.2015 22:56 This SOP describes the application “Easy preparation“ of the PrepGilsonST software, covering the following topics: Add and delete racks (wellplates) manually without barcode reader Create orders for selected source and destination positions in racks with predefined procedures Run preparation for created orders Introduction

3. EasyPreparation without Barcode Reader 3 15.08.2015 22:56 2. Select “Easy preparation“. Click OK. 1. Start PrepGilsonST program with a double click. Program Start and Procedure Selection

4. EasyPreparation without Barcode Reader 4 15.08.2015 22:56 3. Check if Gilson Status is “connected“. Check Connection

5. EasyPreparation without Barcode Reader 5 15.08.2015 22:56 4. Initialize the Gilson® robot by pressing the green button. The status changes to “home seeking“. The Gilson® robot is initialized. Robot Initialization

6. EasyPreparation without Barcode Reader 6 15.08.2015 22:56 5. Select “Edit mode“ to delete or insert racks. Order Setup

7. EasyPreparation without Barcode Reader 7 15.08.2015 22:56 5a. Delete a rack (first the child racks) In “Edit mode“ double click on a gray (not the blue!) area within a rack field. The program asks if the selected rack should be deleted. Click “Yes“. Order Setup

8. EasyPreparation without Barcode Reader 8 15.08.2015 22:56 5a. Delete a rack The child rack is deleted. Order Setup

9. EasyPreparation without Barcode Reader 9 15.08.2015 22:56 5b. Insert a new rack Double click on an empty rack area. Select the rack type used and click “OK“. Order Setup

10. EasyPreparation without Barcode Reader 10 15.08.2015 22:56 5b. Insert a new rack ID Barcode : Enter the new barcode ID, e.g. ABC0033#NMR Type Barcode : Select the Starting corner and pattern. The barcode type is a combination of the rack name, the starting corner and the pattern. Click OK. Order Setup

11. EasyPreparation without Barcode Reader 11 15.08.2015 22:56 5b. Insert a new rack Click “Save Tray“ and uncheck “Edit mode“. Order Setup

12. EasyPreparation without Barcode Reader 12 15.08.2015 22:56 6. Check or select the appropriate procedure and solvent. It is now possible to set up orders. Order Setup

13. EasyPreparation without Barcode Reader 13 15.08.2015 22:56 7. Defining the source positions Click the „Source“ button. Select the source tubes with a lasso operation: Click and hold the left mouse cursor. Draw a rectangle. The marked positions will be shown as orders. Order Setup

14. EasyPreparation without Barcode Reader 14 15.08.2015 22:56 7. Defining the source rack Tip: Use the Ctrl Key and left mouse key to select variable sources. Order Setup

15. EasyPreparation without Barcode Reader 15 15.08.2015 22:56 The marked positions will be shown as orders with their destinations. Select the destination tubes with a lasso operation: Click and hold the left mouse cursor. Draw a rectangle. 8. Defining the destination rack Click the “Destination“ button. Order Setup

16. EasyPreparation without Barcode Reader 16 15.08.2015 22:56 Tip: Use the right mouse key to zoom in and out. Use the Ctrl Key and left mouse key to select variable sources. Order Setup

17. EasyPreparation without Barcode Reader 17 15.08.2015 22:56 9. Click the button “Create Orders“. All “Created Orders“ are listed. Click OK. Orders can be deleted with the button “Delete selected orders“. Order Setup

18. EasyPreparation without Barcode Reader 18 15.08.2015 22:56 10. View the orders running or ready to run. Order Setup

19. EasyPreparation without Barcode Reader 19 15.08.2015 22:56 11. The first order has finished with OK. Order Setup

20. EasyPreparation without Barcode Reader 20 15.08.2015 22:56 12. All orders are finished. Order Setup

21. EasyPreparation without Barcode Reader 21 15.08.2015 22:56