1. Blood Typing Lab
Whodunit?

2. Blood typing What’s your blood type? O+
ABO and Rh are totally independent of each other

4. ABO Antigens and Antibodies
Surface
Antigens
Antibodies A
Anti-B B
Anti-A AB
A, B
none O

5. The Rh Factor Also called D antigen
Either Rh positive (Rh+) or Rh negative (Rh-)
Only sensitized Rh- blood has anti-Rh antibodies
Surface
Antigens
Antibo-dies
Rh+
Rh factor
none
Rh-
Rh-
Sens
none
Anti Rh

7. Blood Types
Genetically determined cell surface markers (antigens) on RBCs, including
ABO group – glycolipids on RBC surface
Rh factor – membrane protein

8. 4 Basic Blood Types A has surface antigen A B has surface antigen B
AB has both antigens A and B
O has neither A nor B
A has type B antibodies
B has type A antibodies
O has both A and B antibodies
AB has neither A nor B antibodies

9. 4 Basic Blood Types Antigens also called agglutinogens
Antibodies called agglutinins
Figure 19–6a

10. ABO Antigens and Antibodies
Surface
Antigens
Antibodies A
Anti-B B
Anti-A AB
A, B
none O

11. The Rh Factor Also called D antigen
Either Rh positive (Rh+) or Rh negative (Rh-)
Only sensitized Rh- blood has anti-Rh antibodies
Surface
Antigens
Antibo-dies
Rh+
Rh factor
none
Rh-
Rh-
Sens
none
Anti Rh

12. Blood type questions
Which blood type is the best in emergency settings (hint: which type can be given to anyone?)
Which blood type is the lucky one that can receive blood from any donor?

13. Cross-Reaction
Figure 19–6b

14. Cross-Reaction If donor and recipient blood types not compatible:
Plasma antibody meets its specific surface antigen and blood will agglutinate and hemolyze

15. Blood Type Test
Determines blood type and compatibility
Figure 19–7

16. Cross-Match Test
Performed on donor and recipient blood for compatibility to blood surface antigens other than ABO and Rh

17. Blood typing procedures
Skip part A
Part B: 6 trays with three wells each
Victim
Crime scene
Suspect 1 A B Rh A B Rh A B Rh
Suspect 2
Suspect 3
Suspect 4 A B Rh A B Rh A B Rh
6 Bottles of “blood” Bottles of antibodies

18. Blood Typing Procedures
For each well Place 1-2 drops of blood from one bottle in all 3 wells
1-2 drops of crime scene in each of the three wells
1-2 drops of victim in each of the three wells
1-2 drops of suspect 1 in each of the three wells
1-2 drops of suspect 2 in each of the three wells
1-2 drops of suspect 3 in each of the three wells
1-2 drops of suspect 4 in in each of the three wells
For each tray add:
1-3 drops of anti-A serum to one well and stir
1-3 drops of anti-B serum to one well and stir
1-3 drops of anti-Rh serum to one well and stir
Look for clumping reaction

19. Blood type? What is the blood type? x = clumping rxn.
Next question: what antibodies does this person have in their blood? A B Rh X X

20. To turn in Lab report pages 5 and 6 with completed table and questions
Due next Thursday