1. PowerPoint Slides to Aid Learning with Forensic DNA Typing Book
Prepared by John M. Butler
©2002 Academic Press
Forensic DNA Typing: The Biology and Technology Behind STR Markers

2. Forensic DNA Typing Book Cover
FORENSIC DNA TYPING Biology and Technology behind STR Markers
John M. Butler ©2002 Academic Press
Published January 2001
Forensic DNA Typing Book Cover

3. Overview and History of DNA Typing
Chapter 1
Overview and History of DNA Typing
©2002 Academic Press

4. Montaret Davis Captured
©2002 Academic Press

5. Human Identity Testing
Forensic cases -- matching suspect with evidence
Paternity testing -- identifying father
Historical investigations
Missing persons investigations
Mass disasters -- putting pieces back together
Military DNA “dog tag”
Convicted felon DNA databases
©2002 Academic Press

6. Progression of DNA Typing Markers
RFLP
multilocus VNTR probes
single locus VNTR probes (32P and chemi)
PCR
DQ-alpha (reverse dot blot)
PolyMarker (6 plex PCR; dots for SNPs)
D1S80 (AMP-FLPs)
singleplex STRs with silver staining
multiplex STRs with fluorescent dyes
©2002 Academic Press

7. Comparison of DNA Typing Technologies
Speed of Analysis (Technology)
Power of Discrimination (Genetics)
Low
High
Slow
Fast
Markers Used (Biology)
RFLP
Single Locus Probes
Multi-Locus Probes
ABO
blood groups
Multiplex STRs
DQ
single STR
D1S80
mtDNA
PolyMarker
©2002 Academic Press

8. Steps in Sample Processing
Sample Obtained from Crime Scene or Paternity Investigation
Biology
DNA
Extraction
DNA
Quantitation
PCR Amplification
of Multiple STR markers
Technology
Separation and Detection of PCR Products
(STR Alleles)
Sample Genotype Determination
Genetics
Comparison of Sample Genotype to Other Sample Results
Generation of Case Report with Probability of Random Match
If match occurs, comparison of DNA profile to population databases
©2002 Academic Press

9. Human Identity Testing Involves Comparing DNA Profiles
gender ID A B C D E F G H I J
DNA Size (base pairs)
probability of a random match: ~1 in 3 trillion
Results obtained in less than 5 hours with a spot of blood the size of a pinhead
Simultaneous Analysis of 10 STRs and Gender ID
©2002 AP, Elsevier

10. Brief History of DNA Typing
Ray White describes first polymorphic RFLP marker
Alec Jeffreys discovers multilocus VNTR probes
first paper on PCR
FBI starts DNA casework
first STR paper
FSS starts UK DNA database
FBI launches CODIS database
©2002 Academic Press