1. GalliPro Bacillus based probiotic

2. Bacillus spore ideal as probiotic for chicken
GalliPro
Comprise live Bacillus spore – natural encapsulated bacteria
Survive feed pelleting process and in feed storage
Has compatibility with all EU approved coccidiostats
Transform from spores to active cells in the intestine of chicken
Reduce FCR
Increase weight gain
Increase nutrient utilization by excretion of enzymes
Improves intestinal health and absorption capacity
Modulate intestinal microflora resulting in more Lactic Acid Bacteria
Reduce Salmonella and Campylobacter

3. Bacillus sp. Definition of Bacillus Spore formers
Gram positive organisms
Spore formers
Bacillus is facultative aerobe but at present of nitrate/nitrite it can grow anaerobic
Bacillus is found in soil and marine sediments
Bacillus as probiotic is in the form of Bacillus spore – a dormant resistant stage that can transform to a vegetative cells
Bacillus spores are
pH stable at pH 2-3
Trypsin stable
Thermo-tolerant
In-Feed stable

4. Bacillus spores the ideal probiotic for chicken?
Pelletizing study on selected probiotic strains on the market
Pelletizing at 85C 30 sec.: External facility, Bioteknologisk Institut, Denmark
Microbiological analysis: External lab, LUFA –ITL-GmbH, Germany
Result: Viable cell count in average CFU/g feed and the recover of viable
Sample ID
Calculated
CFU/g
Meal feed
Pellet feed
Recover of cells after pelleting, %
Control
total aerobic NA
2.6E+06
3.6E+04 1
L.farcimis
3.1E+04
< 10
<1
P.acidilactici
4.0E+06
3.6E+06
2.7E+04
E.faecium
8.5E+06
1.5E+06 18
S.cereviciae
8.0E+06
B.licheniformis
1.3E+06
1.2E+06
100
GalliPro

5. Bacillus spores the ideal probiotic for chicken?
Bacillus spore extremely resistant – easy to apply into feed
Bacillus spore germination occurred rapidly in the GI tracts of chicken (Cartman et al 2008)
Vegetative cells can be detected throughout the GI tract of chicks (Cartman et al 2008)
Feeding Bacillus subtilis to chicken resulted in greater growth performance and intestinal histologies such as increasing villus height. Villus tip showed no damage (Samanya & Yamauchi 2002)
Indicating improved nutrient utilization capacity

6. Bacillus spores the ideal probiotic for chicken?
Feeding GalliPro
More diverse and complex bacteria composition in the gut
Expectedly provides more robust microbiota less susceptible towards diseases and infections (Lan et al 2005)
High numbers of Lactic Acid Bacteria
Considered beneficial due to their putative health properties – protect against pathogens colonization and immune stimulation of the host
Lactobacillus salivarius
Strong probiotic characteristics reducing Salmonella and E. coli (Pascual et al 1999, Brashears et al 2003)
Lactobacillus paracasei
Stimulate the immune system of the host (Herich et al 2002, Morelli et al 2003)

7. GalliPro reduces the level of Campylobacter in broiler chickens
The result shows a significant reduction of Campylobacter by 52% in the GalliPro treated group.
The results on ileal samples showed a 40% reduction of Campylobacter.
Our finding of Campylobacter is accordance with literature. Corry and Atabay (2001) reported the commonly observed level in bird intestinal to be 105 to 107 CFU/g.

8. GalliPro significantly reduce Salmonella in chicken
Salmonella challenge pen floor study (SPR, US) with and without GalliPro showed a significantly reduction of Salmonella in the GalliPro treated group
All samples were found positive at day 42 in the control group whereas only 43% of the samples in the GalliPro group were positive with regards to Salmonella a b
Different letters indication significant difference P< 0.05

9. Bacillus spores the ideal probiotic for chicken?
GalliPro produce enzymes
Alcalin phosphatase
Esterase
Esterase lipase
Lipase
Leucine amino-peptidase
Valine amino–peptidase
Acid phosphatase
Glucosidase
Bacillus shows amylolitic (starch), lipolytic (fat) and proteolytic (protein) activities

10. Summary of 17 trials GalliPro vs. Negative Control
Relative efficacy of GalliPro in broiler trials at independent research institutes
Avg. improvement: Final bodyweight +2,8% & FCR -3,8%
+2,8%
-3,8%

11. Big increases in in-vivo nutrient utilisation
ME 100% diet
Control
GalliPro
Diff
Dry matter ileal digestibility (%)
74,1
78,0
3,9
Crude protein ileal digestibility (%)
82,6
85,6
3,0
Gross energy ileal digestibility (%)
77,0
81,0
4,0
N retention (%)
66,6
68,8
2,2
ME (MJ/kg DM)
13,88
14,19
0,3
ME (MJ/kg DM, N corrected)
13,46
13,76
GalliPro increased in-vivo dry matter, crude protein and gross energy digestibilities, N retention and metabolisable energy.
Study 2 at University Federal de Viçosa, Brazil

12. GalliPro Works! Increased in-vivo DM, CP & GE digestibilities
Increased weight gain, improved FCR and lower mortality
Canadian & EU Registration
Safe, stable and easy to use
Consistent performance

13. Bacillus spores the ideal probiotic for chicken?
Bacillus subtilis (DSM17299) compatibility with Coccidiostats:
Amprolium/ethopabate
Diclazuril
Halofuginone
Lasalocid sodium
Maduramicin ammonium
Methylbenzoquate/meticlorpindol
Monensin sodium
Narasin
Nicarbazin
Nifursol
Robenidine
Salinomycin sodium
Semduramicin