1. Structure and function of biological membranes Biological membranes, together with cytoskeleton, form the structure of living cell. Cell or cytoplasmic membrane surrounds every cell. The nucleus is surrounded by two nucleus membranes external and internal. Biological membranes, together with cytoskeleton, form the structure of living cell. Cell or cytoplasmic membrane surrounds every cell. The nucleus is surrounded by two nucleus membranes external and internal.

2. Structure and function of biological membranes -All the intracellular structures (mitochondria, endoplasmic reticulum, Goldi’ apparatus, lisosomes, peroxisomes, phagosomes, synaptosomes, etc.) represent closed membrane vesicles -Each membrane type contains a specific set of proteins receptors and enzymes but the base of every membrane is a bimolecular layer of lipids ( lipid bilayer ) that performs in each membrane two principal functions: (1) a barrier for ions and molecules

3. Structure and function of biological membranes (2) structural base (matrix) for functioning of receptors and enzymes. The term «membrane» as an invisible film that surround a cell and serves as a barrier between cell contents and the invironment and at the same time as a semipermeable partition through which water and some substances dissolved in it can pass (2) structural base (matrix) for functioning of receptors and enzymes. The term «membrane» as an invisible film that surround a cell and serves as a barrier between cell contents and the invironment and at the same time as a semipermeable partition through which water and some substances dissolved in it can pass

4. Structure and function of biological membranes HISTORY ….. Botanists Negeli in 1855 (explanation of plasmolytic phenomena) …. ….. 1877, Leipzig) postulated the existence of cell membrane Gorter and Grendel showed in 1925 that the area of the monolayer of lipids extracted from erythrocyte membranes is two times larger than the total area of erythrocytes. HISTORY ….. Botanists Negeli in 1855 (explanation of plasmolytic phenomena) …. ….. 1877, Leipzig) postulated the existence of cell membrane Gorter and Grendel showed in 1925 that the area of the monolayer of lipids extracted from erythrocyte membranes is two times larger than the total area of erythrocytes.

5. Structure and function of biological membranes At the same time, there were experimental data that testified to the fact that biological membranes contained protein molecules as part of their composition. These contradictions in experimental results were removed by Danielli & Dawson who proposed in 1935 the so-called «sandwich»(butterbrod/bread-and-butter) model of biological membranes. At the same time, there were experimental data that testified to the fact that biological membranes contained protein molecules as part of their composition. These contradictions in experimental results were removed by Danielli & Dawson who proposed in 1935 the so-called «sandwich»(butterbrod/bread-and-butter) model of biological membranes.

6. Structure and function of biological membranes Electron micrograph of the membranes of two adjacent cells

7. Structure and function of biological membranes Active transport of K +,Na +,Ca 2+, maintaining of osmotic equilibrium Cell (cytoplasmic)All cells Binding of hormones and switching on of mechanisms of intracellular signalling Cell membranesMajority of cells Generation of potentials of peace and action, distribution of action potential Cell membranesNerve and muscle cells

8. Structure and function of biological membranes Transfer of electrons on oxygen and synthesis of ATP (oxidative phosphorylation) Inner membrane of mitochondria Majority of cells (except erythrocytes) Transfer of Ca 2+ from cell juice into vesicles Endoplasmic reticulumMajority of cells (except erythrocytes) Absorption of light quanta and generation of intracellular signal Membranes of eye disksEye epythelium cells

9. Membrane lipids Lipid bilayers are formed by amphiphilic molecules of phospholipids and sphingomyelin in water phase. These molecules are called amphiphilic because they are composed of two parts which differ by their solubility in water: (1) polar «head» possessing high affinity for water, i.e. hydrophilic, and (2).tail» that is formed by non-polar carbohydrate chains of fatty acids; this part of the molecule has low affinity for water, i.e. it is hydrophobic. Fig.2. Membrane lipids are mainly composed of phospholipids, sphingomyelins, and cholesterol

10. Membrane structure Hydrophilic

12. Draw and label a diagram to show the structure of membranes

13. Membrane structure

14. Three categories of membrane protein 1-Integral membrane protein( transmembrane protein) –a. exoplasmic domain hydrophilic cytosolic domain –b. Membrane spanning domain: hydrophobic –c. glycosylated 2. Lipid anchored membrane protein covalently bound to lipid 3. Peripheral membrane protein bound to membrane by interaction with integral membrane protein Three categories of membrane protein 1-Integral membrane protein( transmembrane protein) –a. exoplasmic domain hydrophilic cytosolic domain –b. Membrane spanning domain: hydrophobic –c. glycosylated 2. Lipid anchored membrane protein covalently bound to lipid 3. Peripheral membrane protein bound to membrane by interaction with integral membrane protein

15. - Integral protein: across membrane,and has hydrophobic and hydrophlic part -Lipid anchored: can not across membrane. It bound to one or more lipid molecules. -Peripheral protein: can not interact with the hydrophobic core. Indirectly bound to membrane, via integral protein connect to membrane or cell. May has support Membrane structure