1. BACTERIALBASICS

2. Bacteria Structure Prokaryotes Use asexual reproduction No nucleus
Cell wall
DNA plasmid
(ring-shaped)
Use asexual reproduction

3. Different Forms of DNA Transfer

4. Bacterial Transformation
1928-Frederick Griffith, 1944-Avery, McCarty, Macleod
Process of taking up DNA from media
DNA can be from cells
from same or different
species
DNA will integrate into
the recipient’s
chromosome

5. Bacterial Adhesion Zones
Adhesion zones develop in the bacteria cell membrane, although DNA cannot fit through

6. Calcium Chloride Solution
The addition of calcium ions in CaCl2 solution shields the negatively charged phosphate groups so that the DNA can pass through the membrane when heat shocked.
Heat Shock 42ºC