1. OnSite Rapid Test Procedure Training
Part 2
CTK-MK-PPT-R0001 (2)

2. Disclaimer
This is a general training presentation based on the OnSite Rapid Test procedure. Trainees should take precautions when performing a specific assay, and should strictly follow the procedure provided by the test kit.

3. Training Contents Introduction Perform Assay Before, During, After 12
Perform Assay Before, During, After 3
Assaying with Accuracy 4
Troubleshooting 5
Product Evaluation
Additional Information
Storage Temperature & Shelf Life 6

4. Before Assay Check storage room temperature daily
Check inventory and test kit lots if needed
Store at 2-30 ℃, sealed

5. Test results log sheets
Before Assay
Receive request for testing
Check testing room temperature
Recommended 15-30℃
Prepare the control and test results log sheets
Test results log sheets

6. Before Assay Perform external quality control if needed
Check product’s name and expiration date
Product Name
Expiration Date

7. Before Assay
Read the package insert and other provided information

8. Before Assay Set up testing area Set up Testing Area Purpose
Cleanliness
Avoid mix-ups
Clock, timer, or watch
Count reaction time
Disposable glovers
Biohazard precaution
Biohazard disposal container
Dispose used device, dropper, tips specimen container
Marker
Label device
Bright light
Easy to read result

9. Before Assay Follow biohazard safety precautions Wear lab coat
Wear gloves
Use protection pad for strip tests

10. Before Assay Serological specimen preparation Collection Storage
No abnormal specimens
Whole Blood
Plasma
Serum
At 2-8°C for specific defined period
At -20°C for long term storage
Blood Abnormality
Influence
Hemolyzed
Background
High lipid
Flow and background
Cloudy (Bacteria)
Incorrect result
Precipitants
Flow and result

11. Nasal/Nasopharyngeal aspirate
Before Assay
Respiratory specimen preparation
Collection
Extraction
Nasal swab
Throat swab
Nasopharyngeal swab
Nasal/Nasopharyngeal aspirate
or wash specimen
Storage
At 1-8°C for specific defined period
At -20°C for long term storage

12. Before Assay Fecal specimen preparation Collection and extraction
Transportation and storage
At 2-8°C for specific defined period
At ≤ -20°C for long-term

13. Collect urine sample randomly Label a clean urine container
Before Assay
Urine specimen preparation
Collection
Transportation and storage
Collect urine sample randomly
Label a clean urine container
At 2-8°C for specific defined period
At ≤ -20°C for long-term

14. Before Assay Genital specimen preparation Collection
Follow standard procedures
Extraction
Transportation and storage
At room temperature for specific defined period

15. Before Assay Specimen preparation
Bring pre-prepared specimen to room temperature
Check specimen for abnormality

16. Cassette device in pouch Strip device in canister
During Assay
Open the pouch or canister immediately prior to testing
Cassette device in pouch
Strip device in pouch
Strip device in canister

17. During Assay Label the test device or specimen
The strips in the canister MUST be used within days from the date it is first opened
Label cassette device
Label the canister
For dip-strip device, label the specimen container
For non-dip strip device, flatten strip and label on the paper

18. During Assay Perform the test on the device Load specimens
Load sample diluent
(as applicable)
Read result in defined time
Cassette
Non-dip strip
Dip strip

19. During Assay: Result Interpretation
Sit on the working stool
Keep test device on table/lab bench
Look straight down from above test device under bright light
Observe test result around 5 seconds
Take picture if needed
Negative
Positive
Invalid

20. After Assay Document the result Report to supervisor if necessary
Clean up and dispose of biohazard waste
Participate in periodic external quality assessment
Specimen C T
Result
ID xxx + -
Negative
Positive
Invalid

21. After Assay
If necessary, request confirmatory test according to facility instruction
Collect, process and transport confirmatory test specimens
Maintain confidentiality
Manage confirmatory test result