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Optoelectronics & Microsystems Lab Politecnico di Milano, Dip. Elettronica e Informazione, Milano, Italy.

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Presentation on theme: "Optoelectronics & Microsystems Lab Politecnico di Milano, Dip. Elettronica e Informazione, Milano, Italy."— Presentation transcript:

1 Optoelectronics & Microsystems Lab Politecnico di Milano, Dip. Elettronica e Informazione, Milano, Italy

2 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab STAFF COVA, S. Full Professor GHIONI, M. Full Professor ZAPPA, F. Associate Professor GIUDICE, A. Post-Doc Research Associate RECH, I. Post-Doc Research Associate LABANCA, I. Research Associate GALLIVANONI, A.Research Associate TISA, S.Ph.D. Student RESTELLI, A. Ph.D. Student TOSI, A.Ph.D. Student GULINATTI, A. Ph.D. Student

3 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Core Know-How Microelectronic Detectors and Circuits Measurement Techniques and Instrumentation for detecting and measuring optical signals with ultra-high sensitivity and picosecond resolution

4 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Recent Achievement Ultrasensitive CE-Microchip apparatus for DNA fragment separation collaboration with M. Chiari, CNR Inst. of Chemistry and Molecular Recognition, Milano Reference: I. Rech, A. Restelli, S. Cova, M. Ghioni, M. Chiari, ad M. Cretich, Microelectronic Photosensors for Genetic Diagnostic Microsystems Journal of Sensors and Actuators – B, in press 2004

5 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Separation Injection Sample Buffer Waste Chip Electrophoresis

6 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab S/N=35 CE Separation in glass microchip run buffer: TAPS-TRIS 100mM pH 8.5. Sample : 23 mer oligonucleotide labelled with CY 5 Detection limit S/N=3) : 1pM corresponds to < 30 molecules in the injection volume of 50pL

7 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Looking inside the apparatus... Compact & low-cost set-up with: Fully automated operation Laser diode excitation of fluorescence Controlled chip temperature Dual wavelength detection Dual HV power supply (0-5kV) Confocal optical scheme Remote control Problem debug via internet

8 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Looking the apparatus at work in the lab...

9 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Recent Achievement Microelectronic Single-Photon Detector for studies on Single-Molecule Dynamics collaboration with X. Sunney Xie, Harvard University, Dept. of Chemistry and Chemical Biology Reference: H. Yang, G. Luo, P. Karnchanaphanurach, T.M. Louie, I. Rech, S.Cova, L. Xun, and X. Sunney Xie, Single-Molecule Protein Dynamics on Multiple Time Scales Probed by Electron Transfer, SCIENCE, Oct.10, 2003, Vol.302,

10 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Xies idea: Measuring the delay of the fluorescence photon with respect to the laser excitation with picosecond-resolution, good efficiency and low-noise makes possible to probe the Single-Molecule Protein Dynamics by means of a correlation analysis of the delay fluctuations in real time However: even the best commercially available Photon Counting Modules DO NOT provide adequate performance!

11 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab SPTM Single Photon Timing Module for Single-Molecule Experiments FWHM=60ps High Time-resolution: 60ps Low Dark Counts: down to 5 c/s Quantum Detection Efficiency: 500nm Single power supply +15V Controlled Temperature (Peltier cell) Software controlled settings RS-232 data transmission

12 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Recent Achievement Non-invasive testing of ULSI Circuits by Ultrasensitive Picosecond Time-Resolved Luminescence Measurements collaboration with IBM T.J. Watson Research Center, Yorktown Heights, NY Reference: Stellari F., Zappa F., Cova S., Porta C., Tsang J.C., "High-Speed CMOS Circuit Testing by 50ps Time-Resolved Luminescence Measurements" IEEE Trans. Electron Devices, 48, (2001)

13 S. Cova et al., 2004 POLIMI - Politecnico di Milano, DEI Optoelectronics & Microsystems Lab Recent Achievement Photon-Counting Quadrant Detectors and Electronics for Adaptive Optics Systems of Advanced Telescopes collaboration with D.Bonaccini, ESO European Southern Observatory, Garching, D and R. Biasi, Microgate, Bolzano, I Reference: development of the STRAP (System for Tip-tilt Removal with Avalanche Photodiodes) for the VLT Observatory, Cerro Paranal, Chile


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