1. ALZET Speakers Kit
ALZET Osmotic Pumps

2. ALZET Osmotic Pump Advantages
Ensure around-the-clock exposure to test agents at predictable levels
Permit continuous administration of short half-life proteins and peptides
Convenient method for chronic dosing of laboratory animals
Minimize unwanted experimental variables and ensure reproducible, consistent results
Eliminate the need for nighttime or weekend dosing
Reduce handling and stress to laboratory animals
Small enough for use in mice or very young rats
Allow for targeted delivery of agents to virtually any tissue
Cost-effective research tool
ALZET Osmotic Pumps

3. Injection vs. Infusion
ALZET Osmotic Pumps

4. Principle of Operation
ALZET pumps have 3 concentric layers:
Rate-controlling, semi-permeable membrane
Osmotic layer
Impermeable drug reservoir
ALZET pumps work by osmotic displacement. Water enters the pump across the outer, semi-permeable membrane due to the presence of a high concentration of sodium chloride in the osmotic chamber. The entry of water causes the osmotic chamber to expand, thereby compressing the flexible reservoir and delivering the drug solution through the delivery portal.
ALZET Osmotic Pumps

5. ALZET Osmotic Pumps

6. ALZET® osmotic pumps are available in three sizes

7. ALZET Brain Infusion Kits
Together with ALZET pumps, the Brain Infusion Kits permit the continuous delivery of test compounds to the CNS for extended periods of time without external connections, restraints, or frequent handling of laboratory animals.
ALZET Osmotic Pumps

8. Specifications of ALZET Brain Kits
Brain Infusion Cannula
Material (tube)
Stainless steel
Dimensions (steel tube)
28 gauge
30 gauge
Material (elbow stop, flange)
ID = 0.18 mm; OD = 0.36 mm
ID = 0.16 mm; OD = 0.31 mm
Polycarbonate
Volume inside tube
0.32 µl
0.23µl
Height Adjustment Spacers
Material
Dimensions (height)
0.5 mm
Catheter Tubing
Polyvinylchloride (Medical grade)
Length
15 cm (approx.)
Inside diameter
0.69 mm (± 0.08)
Outside diameter
1.14 mm (± 0.08)
Volume per 15 cm
56 µl (3.7 µl/cm)
ALZET Osmotic Pumps

9. ALZET Brain Infusion Kits
ALZET Brain Infusion Kits are designed for targeted delivery to the cerebral ventricles or direct microperfusion of discrete brain structures. Available kits have slightly different cannula designs.
ALZET Osmotic Pumps

10. In vitro vs. in vivo pumping rate
The typical pumping rate of the Model 2ML4 ALZET Osmotic Pump over time in Sprague Dawley rats after subcutaneous implantation (n=105)
ALZET Osmotic Pumps

11. Typical pumping rate of the Model 2002 ALZET Osmotic Pump over time (n-20)
ALZET Osmotic Pumps

12. ALZET Contact Information
ALZET Customer Service
(Pricing and Ordering)
Toll free:
Phone:
Fax:
Mailing Address
DURECT Corporation
P.O. Box 530
Cupertino, CA
ALZET Technical Support
Toll free:
Phone:
Fax:
Web Address
ALZET Osmotic Pumps