1. Sequencing activities in EU-SOL René Klein Lankhorst PAG 2007

2. Resources & Goals Budget entire EU-SOL Project: 25.9 M€ (34.5 M$) Work Package 5.4, Tomato genome sequencing (René Klein Lankhorst, Wageningen University) –Budget: 2 M€ –Goals: De-bottlenecking current European tomato sequencing programs Sequencing the tomato mitochondrial genome (INTA) Development of novel technologies for BAC assembly and closure (Genome Express)

3. Resources & Goals, continued Work Package 6.1, Tomato genome bioinformatics (Yves van de Peer, VIB Gent) –Budget: 1.3 M€ –Goals: Train and run a full-featured annotation pipeline Build a database for storage of BAC annotations Further improvement of the EuGene prediction and annotation platform Further development of genome assembly pipelines

4. Resources & Goals, continued Work Package 6.3, Integrated Solanaceae data resource and analysis (Heiko Schoof, Cologne) –Budget: 1.2 M€ –Goals (amongst others): Develop tools for visualization, analysis and data mining Set up a distributed data resource and analysis platform Link the EU-SOL data resource to the international Solanaceae data resources

5. Currently approved proposals for de-bottlenecking Italy: –FISH validation of seed-BACs* –outsourcing of BAC-sequencing ( up to Phase III) Spain: –Identification of novel seed-BACs –FISH validation of seed-BACs* –outsourcing of BAC-sequencing ( up to Phase I) Netherlands: –Closure of ~200 BACs * Hans de Jong (Wageningen) will provide FISH-services to all European countries

6. Novel markers and seed-BACs A request was made to the following EU-SOL members: Syngenta Keygene De Ruiter Seeds Rijk Zwaan ENZA Vilmorin