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Instructions for using this template. Remember this is Jeopardy, so where I have written “ Answer ” this is the prompt the students will see, and where.

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Presentation on theme: "Instructions for using this template. Remember this is Jeopardy, so where I have written “ Answer ” this is the prompt the students will see, and where."— Presentation transcript:

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2 Instructions for using this template. Remember this is Jeopardy, so where I have written “ Answer ” this is the prompt the students will see, and where I have “ Question ” should be the student ’ s response. To enter your questions and answers, click once on the text on the slide, then highlight and just type over what ’ s there to replace it. If you hit Delete or Backspace, it sometimes makes the text box disappear. When clicking on the slide to move to the next appropriate slide, be sure you see the hand, not the arrow. (If you put your cursor over a text box, it will be an arrow and WILL NOT take you to the right location.)

3 Choose a category. You will be given a question. You must give the correct answer. Click to begin.

4 Click here for Final Jeopardy

5 ELISA Medical Interventions Outbreaks Immune System Vocabulary 10 Point 20 Points 30 Points 40 Points 50 Points 10 Point 20 Points 30 Points 40 Points 50 Points 30 Points 40 Points 50 Points Serial Dilutions

6 What does ELISA stand for?

7 Enzyme-Linked Immunosorbant Serological Assay

8 What are the 2 types of ELISA tests?

9 Antigen Detection ELISA & Antibody Detection ELISA

10 What is significant about the secondary antibody created for the ELISA test?

11 The secondary antibody: Is created to specifically bind the primary antibody It is conjugated with an enzyme

12 What reaction occurs in an ELISA, causing a visible color change?

13 The enzyme oxidizes the substrate?

14 Why are antibodies used to identify the presence of a particular antigen?

15 Antibodies are only created in response to a specific antigen and will only bind that one antigen

16 What is a medical intervention?

17 Any measure that improves health or alters the course of a disease.

18 List 3 interventions that could be documented for Sue Smith

19 Inhaler Ibuprofen Pathogen DNA sequencing ELISA Urine, blood or lymph samples Antibiotics for N. meningitidis

20 List 3 major medical intervention categories

21 Diagnostics Surgery Medications rehabilitation Genetics Immunology Medical devices

22 Which medical intervention category does an ELISA fall into?

23 diagnostics

24 List 3 interventions that could be used for a broken leg.

25 Xray Pain meds Cast Crutches/wheel chair surgery

26 What is the liquid used to dilute a substance called?

27 diluent

28 How is a tube dilution calculated?

29 Amount of sample _________________ Total volume in tube

30 Calculate the tube dilution for the following tube:

31 1/10

32 Calculate the final dilution for tube #4

33 1 / 10,000

34 If tube #1 has a concentration of 100 ug/ml, what is the concentration in tube #3?

35 1 ug/ml

36 What is an outbreak?

37 A rise in disease occurrence in an area

38 List 4 steps taken by scientists during an outbreak

39 Interview patients Symptoms tracker Patient connections web Pathogen DNA sequencing ELISA Treat Stop spread Prevent future spread

40 What is the best medical intervention to determine patient zero?

41 Quantitative concentration data from an ELISA.

42 How do scientists determine the pathogen responsible for the outbreak?

43 Pathogen DNA isolation and sequencing

44 Why is it important for doctors to know the concentration of pathogen in patients during an outbreak? 2 reasons

45 1.Determine patient zero 2. Track the path of infection

46 Which immune system structure produces antibodies?

47 B cells

48 How many antigens will an antibody bind?

49 Only one

50 What is an antigen?

51 An identifying protein marker on the surface of cells

52 What does the presence of the specific antibody for HIV in a patient’s system indicate?

53 That patient has encountered the HIV virus in their body

54 Why is antigen concentration alone sometimes not enough to determine patient zero?

55 A patient’s immune system may have already reduced the amount of pathogen present

56 What is the collection, classification, storage, and analysis of biochemical and biological information using computers especially as applied in molecular genetics and genomics

57 Bioinformatics

58 What is a specific causative agent of disease?

59 Pathogen

60 The amount of a specified substance in a unit amount of another substance

61 concentration

62 What is an antigen-binding immunoglobulin, produced by B cells, that functions as the effector in an immune response

63 Antibody

64 What is a foreign macromolecule (protein) that does not belong to the host organism and elicits an immune response?

65 An antigen

66 Make your wager

67 How does a visible color change in an antigen detection ELISA prove the presence of the antigen?

68 The enzyme connected to the secondary antibody oxidizes the substrate to produce a color change. The secondary antibody is not present unless the primary antibody is present. The primary antibody is not present unless the antigen is present. Therefore, the presence of the secondary antibody proves the presence of the antigen.

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Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

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Instructions for using this template. To enter your questions and answers, click once on the text on the slide. Then highlight and just type over what’s.

Instructions for using this template. To enter your questions and answers, click once on the text on the slide. Then highlight and just type over what’s.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.
Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

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Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

Instructions for using this template. Remember this is Jeopardy, so where I have written “Answer” this is the prompt the students will see, and where.

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