1. Chapter 5 Protein Function Copyright © 2011 by John Wiley & Sons, Inc. Charlotte W. Pratt | Kathleen Cornely Essential Biochemistry Second Edition

3. N-terminus C-terminus

4. F8 His

5. Y = Fractional Saturation (Y=1 for 100% saturation) p 50 = 2.8 torr

6. Mb α β

7. Amino Acid Sequences of Mb and Hb Residues identical in α and β subunits Residues identicl in all 3 Residues invariant in all vertebrates

8. Globin evolution

9. Mb Hb p 50 Hb = 26 torr p 50 Mb = 2.8 torr

10. Hb confomation changes upon oxygenation/deoxygenation

11. Figure 7-5

12. DeoxyHb OxyHb

13. Figure 10-16Triggering mechanism for the T  R transition in Hb. Page 333

14. Subunit interactions change deoxygenation (b) deoxygenation oxygenation

15. Figure 10-17The  1 C–  2 FG interface of Hb in (a) the T state and (b) the R state. Page 334

16. Figure 10-18The hemoglobin  1  2 interface as viewed perpendicularly to Fig. 10-13. Page 335

17. Box 7-3

18. Figure 7-15

19. Figure 7-16

20. Table 7-1

21. A map indicating the regions of the world where malaria caused by P. falciparum was prevalent before 1930. Page 184

23. Linus Pauling noted that sHb had a different charge than normal Hb. Single point mutation at position A3 in the β subunit: Glu →Val!!!

24. Figure 7-19

25. SHb polymers

26. Figure 7-18b

28. Tissue Lungs

29. Figure 10-7Comparison of the O 2 - dissociation curves of “stripped” Hb and whole blood in 0.01M NaCl at pH 7.0. Page 326

30. Binding and Release of: O 2, BPG, CO 2, H + 4O 2 + Hb-BPG-CO 2 -H + Hb-(O 2 ) 4 + BPG + CO 2 +H + BPG + CO 2 +H + + Hb-(O 2 ) 4 Hb-BPG-CO 2 -H + + 4O 2 Lungs: Capillaries: Respiring cells: O 2 +MbMb-O 2 Mitochondrion(O 2 ) + Mb CO 2

33. Binding of BPG to deoxyHb.

35. The effects of BPG and CO 2, both separately and combined, on hemoglobin’s O 2 -dissociation curve compared with that of whole blood (red curve).

37. Fibrous proteins Actin and Tubulin

38. Actin monomer ATP ATP is hydrolyzed to ADP to drive the polymerization reaction

40. F-actin (not G-actin catalyze ATP hydrolysis

41. Polymerization is reversible

43. Microfilaments help cells move

44. β-Tubulin Tubulin αβ dimer with bound G

45. Assembly of a microtubule

46. Tubulin depolymerization Protofilaments curve away from the microtubule and separate before dimers dissociate

47. Microtubules in a dividing cell!

48. Product of the meadow saffron plant causes microtubules to depolymerize and therefore blocks cell division!

49. Originally from the endangered Pacific yew tree Binds β tubulin in a microtubule but not to free tubulin preventing depolymerization and thus cell division. Toxic to rapidly dividing cells.

50. SEM of Human skin!!!

51. Keratin forms a coil-coil structure.

54. HAIR

55. Collagen

56. Collagen: mostly (Gly-Pro-Hyp) n

57. Narrow lh helix Pro Hyp Gly

58. Collagen triple helix

59. Cross-linking of collagen fibers Cross-linking increases with age—skin is less plastic

60. A CHILD with a collagen defect Osteogenisis imperfecta

61. Myosin

63. Thick filaments of Myosin