1. Mitosis n Mitosis is the process by which new cells are generated. n Mitosis produces 2 genetically identical daughter cells

2. Parent cell Replicated chromosomes condense at the start of mitosis Mitosis Sister chromatids separate and two daughter cells are formed. Parent cell and daughter cell contain the same complement of chromosomes. Overview of Mitosis:

3. The cell cycle:

4. Figure 12.5 The stages of mitotic cell division in an animal cell: G 2 phase; prophase; prometaphase

5. Figure 12.5 The stages of mitotic cell division in an animal cell: metaphase; anaphase; telophase and cytokinesis.

6. Figure 12.5x Mitosis

7. How do spindle fibers move the chromosomes?

8. The kinetochore is a specialized region on the centromere where spindle fibers attach

9. Motor molecules of the kintochore walk along the microtubule, taking the chromosome with it As the chromosomes move toward the poles, the microtubules are broken down at the kinetochore end

10. Transport vesicle Kinesin Microtubule ATPADP+P i Motor molecules "walk" along a microtubule track ATPADP+P i

11. After the nucleus divides, cytokinesis divides the cytoplasm, forming two separate cells

12. Cytokinesis in animals Figure 8.9a Cleavage furrow

13. Mitosis in a plant cell

14. Bacteria divide by binary fission

17. Unregulated cell division can lead to cancer n Many factors interact with each other to determine whether a cell will undergo mitosis n Cell cycle checkpoints normally insure that DNA replication and mitosis occur only when conditions are favorable and the process is working correctly.

18. G 2 checkpoint Pass this checkpoint if: cell size is adequate chromosome replication is successfully completed M G2G2 Mitosis Metaphase checkpoint Pass this checkpoint if: all chromosomes are attached to mitotic spindle G1G1 First gap S DNA synthesis G 1 checkpoint Pass this checkpoint if: cell size is adequate nutrient availability is sufficient growth factors (signals from other cells) are present Second gap Cell-Cycle Checkpoints

19. Cell cycle proteins regulate whether a cell will proceed past a particular cell cycle checkpoint cyclin - a protein whose amount varies cyclically cdk - another cell division control protein - a cyclin dependent kinase; active only when bound to cyclin MPF - mitotic promoting factor... MPF is a kinase enzyme, ones that switches on/off target proteins by phosphorylating them..... [cyclin + cdk = MPF]... favors Mitosis leads to destruction of cyclin itself

20. MPF levels rise, causing the cell to pass through the G2 checkpoint

21. Growth factor proteins are signal molecules that trigger a signal- transduction pathway leading to cell division Platelet-derived growth factor

22. Cell division is also dependent on environmental factors

23. Unregulated cell division can lead to cancer n Mutations in genes that encode cell cycle proteins can lead to unregulated growth, resulting in tumor formation and ultimately invasion of cancerous cells to other organs.

24. Cancer cells have escaped cell cycle controls and divide excessively

25. Figure 12-17x1 Breast cancer cell

26. Figure 12-17x2 Mammogram: normal (left) and cancerous (right)

27. CANCER SPREADS