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Arsenite oxidase, an ancient bioenergetic enzyme?.

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Presentation on theme: "Arsenite oxidase, an ancient bioenergetic enzyme?."— Presentation transcript:

1 Arsenite oxidase, an ancient bioenergetic enzyme?

2 ATPase Electron transfer chain H+H+

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6 Hélice transmembranaire Cluster [2Fe-2S] Domaine soluble

7 Ellis et al Structure 9, arsenite oxidase from Alcaligenes faecalis

8 Distribution of arsenite oxidases on the phylo tree of species

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10 ssu-rRNA:

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12 Chloroflexus aurantiacus - thermophilic photosynthetic bacterium (affiliated to Thermus-group) - isolated from a Yellowstone Park hot spring - earliest branching phototroph in standard ssu rRNA-trees - genome sequenced no bc-complex in Chloroflexus (genome), sole Rieske protein in the arsenite oxidase operon Inital results: - Rieske protein is membrane-associated (no RPE signal in supernatant) - Rieske protein can be light-oxidised, i.e. electrons from As III oxidation enter the photosynthetic electron transfer chain

13 ATPase Electron transfer chain H+H+

14 ATPase H+H+ Chloroflexus in the presence of arsenite h arsox AuCy mena- quinone ? As III As V photo- system + -

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16 Mo ? [3Fe4S] Rieske AuCy P/P + (photosystem) E m (mV) ? 300 Mo [3Fe4S] Rieske cyt c oxidase Alcaligenes faecalis As III / As V Chloroflexus aurantiacus ?

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18 BIP 09/CNRS Marseile Frauke Baymann (CR1) Anne-Lise Ducluzeau (Mastère) Simon Duval (Mastère) Evelyne Lebrun (CR1) Wolfgang Nitschke (DR2) Barbara Schoepp (CR1)


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