1. RNA Backbone Rotamers: Introduction and Updates Laura Weston Murray July 27, 2008 ROC Meeting, Berlin

2. Outline Background information on RNA backbone consensus conformers Applications and collaborations Dataset update (in progress)

3. A Series of Tubes 1Q9A 1JJ2

6. Classification Consensus Selected and quality-filtered dataset Nomenclature to translate among residue, dinucleotide, suite descriptions Applied and cross-referenced methods –7D visualization –Pseudo-electron density contours –Multi-dimensional binning –Pseudotorsion analysis

7. Selection and Quality Filtering Dataset selection –resolution (3Å and better) –variety B-factor –local reliability Sterics –Eliminate the impossible

10. 1a #a 4s 5z 1a 1e 1a

11. Applications Motif identification and description –But Gary will tell you more about that one. PHENIX RNABC Ribosome

12. Applications Motif identification and description –But Gary will tell you more about that one. PHENIX RNABC Ribosome

14. Applications Motif identification and description –But Gary will tell you more about that one. PHENIX RNABC Ribosome

15. RNABC: RNA Backbone Correction

16. Original  dihedral RNABC conformation RNABC correction 50S 1941-2

17. Applications Motif identification and description –But Gary will tell you more about that one. PHENIX RNABC Ribosome

19. RNA08 RNA03RNA05RNA08 est. Files132171200 Residues8636948615000

20. Acknowledgements Richardson LabRutgers-PragueGeorgia TechYale Jane RichardsonHelen BermanLoren WilliamsAnna-Marie Pyle David RichardsonBohdan SchneiderEli HershkovitzJohn Westbrook Gary KapralZdenek MoravekKevin Keating Bryan ArendallDavid Micallef Lizbeth VideauDaniela Hamova Ian Davis Funding NIHHHMINSFROC