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Division cellulaire asymétrique A B M. Neuron Sheath Shaft Socket.

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Presentation on theme: "Division cellulaire asymétrique A B M. Neuron Sheath Shaft Socket."— Presentation transcript:

1 Division cellulaire asymétrique A B M

2

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4 Neuron Sheath Shaft Socket

5 pI pIIb pIIa métaphase télophase A101 / -tub / Numb pI pIIb pIIa pIIIb Glial Neurone Sheath Shaft Socket

6 Répartition inégale des déterminants de lidentité cellulaire AB 1. Polarisation 2. Distribution asymétrique des déterminants 3. Position du site de clivage (répartition inégale)

7 AA 1. Perte de polarité2. Défaut dans la position du site de clivage AA

8 Knoblich, 2002

9 pI pIIb pIIa métaphase télophase A101 / -tub / Numb pI pIIb pIIa pIIIb Glial Neurone Sheath Shaft Socket

10 pI pIIb pIIa pIIIb Glial Neurone Sheath Shaft Socket pI pIIa Socket pI pIIb Neurone/Glial wild-type numb Notch-Act Notch

11 wild-type numb Notch (ts) Notch (ts) + numb Numb Notch Jan, 1996

12 -adaptin

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14 Numb -adaptin pI pIIa pI pIIb -adaptin Hs-numb

15 -adaptin Notch pI pIIa pI pIIb -adaptin Notch (ts) - 29°C

16 Numb -adaptin Notch Numb pIIa pIIb pIIa Notch

17 Pon-GFP

18 pI epidermal Pon-GFP Histone2B-YFP anteriorposterior apical basal

19 Fz Dsh Pk Stbm Planar polarity in Drosophila frizzled flamingo strabismus dishevelled prickle

20 Polar distribution of planar polarity proteins Fz Pk, Stbm Fz-GFP pI Stbm H2B-YFP GFP-Stbm H2B-YFP

21 Planar polarity genes orient pI cells -tubulin Numb frizzled flamingo strabismus dishevelled prickle wild-type random

22 posterioranterior Fz, Dsh orientation (prior to mitosis) Stbm, Pk Numb ?

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24 Les neuroblastes de lembryon de Drosophile Temps VNE neuroblaste épiderme GCM neurones

25 Le déterminant Prospero Neuroblaste spécifique : dpn,ase GMC spécifique : eve,ftz sauvageprospero

26 ProsperoMiranda Nbs GMC

27 The mitotic spindle rotates by 0-90° - Centrosomes are randomly positioned at separation - Centrosomes migrate by the shortest path to orient the spindle No evidence for centrosome priming

28 Identification du gène inscuteable

29 Inscuteable se localise au pôle apical interphaseprophasemétaphaseanaphase

30 Inscuteable est requis pour localiser Miranda Miranda / ADN Insc -

31 Baz DmPKC DmPar6 Inscuteable -Localisation de Miranda -Rotation du fuseau G i/Gß Baz DmPKC DmPar6 Inscuteable Pins-G iGDP Cibles?

32 apical cue Bazooka (PAR-3) DmPAR-6 DaPKC Inscuteable Pins Mitotic spindle rotation Basal accumulation of Numb and Prospero Drosophila neuroblasts G i-GDP + G Chia, Doe, Jan, Knoblich, Knust...

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34 Fz Dsh Pk Stbm Planar polarity in Drosophila frizzled flamingo strabismus dishevelled prickle

35 Planar polarity genes orient pI cells -tubulin Numb frizzled flamingo strabismus dishevelled prickle wild-type random

36 Baz PAR-6 aPKC Pins G i- GDP Numb G effectors aPKC targets baz wild-typepins Numb pins bazooka and pins are required to localize Numb... Numb

37 Establishment of polarity in pI cells and in neuroblasts Planar polarity signal Dlg-Pins (anterior) Bazooka DaPKC DmPAR-6 (posterior) pINeuroblast Apical signal Bazooka DaPKC DmPAR-6 (apical) PinsInscuteable Numb localises opposite to Baz

38 Inscuteable reverts pI cell polarity WT Numb Insc Numb Pins WT UAS-Insc Insc Numb Pins BazDlg UAS-Insc Dlg-Pins Bazooka DaPKC DmPAR-6 (anterior) Numb (posterior) Inscuteable

39 Numb (drosophile-mammifères: 603 aa) PTB Neurorétine


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