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Investigation of the BARREN STALK1 gene lineage in graminoid Poales Daniel Woods.

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Presentation on theme: "Investigation of the BARREN STALK1 gene lineage in graminoid Poales Daniel Woods."— Presentation transcript:

1 Investigation of the BARREN STALK1 gene lineage in graminoid Poales Daniel Woods

2 rice corn

3 Outline 1)Introduction into field of Evolutionary Developmental Genetics and Grasses 2)What’s BARREN STALK1 3) Hypothesis/aims 4) Preliminary Data

4 rice corn Graminoid Poales ? phylogenetics morphological development molecular evolution functional analyses gene expression

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7 Chasmanthium (CEN) inflorescence development

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9 barren stalk1 (ba1) phenotype Wild-type tasselba1-ref WT meristem ba1-mum1 Gallavotti, A., et al. (2004). Nature 432(7017): 630-5

10 Rice (OsLAX) Maize (ZmBA1) BA1/LAX expression via in situ hybridization Gallavotti, A., et al. (2004), Nature 432(7017): 630-5 Komatsu, K., et al. (2003). Proc Natl Acad Sci USA 100(20): 11765-70

11 Hypotheses: 1)BA1 duplication event occurred at base of grass family 2)BA1 is expressed in branching primordia throughout inflorescence development

12 Specific aims to test hypothesis 1)Isolate BA1 bHLH genes from diverse array of grasses and near grass relatives 2) Generate phylogenies to pinpoint duplication event 3) Document inflorescence development using Scanning Electron Microscopy (SEM) 4) Gene Expression Analysis via in situ hybridization

13 BEP PACCMAD

14 Gene Isolation and Preparation Extractions Primer Design Polymerase Chain Reaction(Semi-nested) Gel Extraction Ligation Clone PCR screen Alkaline lysis Sequence(Sanger)

15 Generate Phylogenies Maximum Parsimony Maximum Likelihood Bayesian

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