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Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein expression. 2.mRNA protein degradation. 3.mRNA secondary structure.

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Presentation on theme: "Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein expression. 2.mRNA protein degradation. 3.mRNA secondary structure."— Presentation transcript:

1 Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein expression. 2.mRNA protein degradation. 3.mRNA secondary structure. 4.Missing factors in E.coli S-30 extract

2 100 75 50 37 25 15 10 S1 S2 S4 S5 S13? S16? S?

3 “Specialized” ribosome GFP X W X W Ribosome N N S N AGUGTAU UCACAUA

4 Future Steps and Issues 1.Increase translation efficiency. 2.Reconstitute 30S and 50S subunits. 3.Genome organization. 4.Autogenous regulation. 4. RNA processing & modifications.

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