1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 9, part B Biotechnology and Recombinant DNA

2. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Genetic Engineering Figure 9.11.1

3. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Genetic Engineering Figure 9.11.2

4. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Making a Gene Product Figure 9.12.1

5. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Making a Gene Product Figure 9.12.2

6. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Used because it is easily grown and its genomics are known Need to eliminate endotoxin from products Cells must be lysed to get product E. coli

7. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Making a Gene Product Figure 9.13

8. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Subunit vaccines Nonpathogenic viruses carrying genes for pathogen's antigens as vaccines Gene therapy to replace defective or missing genes Human Genome Project Nucleotides have been sequenced Human Proteome Project may provide diagnostics and treatments Therapeutic Applications

9. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Random Shotgun Sequencing Figure 9.14

10. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Understanding of DNA Sequencing organisms' genomes DNA fingerprinting for identification Scientific Applications Figure 9.16

11. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Southern Blotting Figure 9.15.1

12. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Southern Blotting Figure 9.15.2

13. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Southern Blotting Figure 9.15.3

14. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Agricultural Applications Table 9.2

15. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Genetic Engineering Using Agrobacterium Figure 9.18

16. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Avoid accidental release Genetically modified crops must be safe for consumption and for the environment Who will have access to an individual's genetic information? Safety Issues and Ethics