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DA dendrite formation. Dendrite guidance Clock model: axonal and dendritic growth are internally regulated, depending on how to respond to guidance molecules.

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Presentation on theme: "DA dendrite formation. Dendrite guidance Clock model: axonal and dendritic growth are internally regulated, depending on how to respond to guidance molecules."— Presentation transcript:

1 DA dendrite formation

2 Dendrite guidance

3 Clock model: axonal and dendritic growth are internally regulated, depending on how to respond to guidance molecules

4 WT Robo3 over- expression in TTMn Robo3 over- expression in both GF & TTMn GF, giant fiber interneuron TTMn: tergotrochantral motor neuron

5 Neuropil model: same guidance molecules for attracting (or including) axon and dendrite together for synapse TRENDS in Neurosciences Vol.27 No.4 April 2004

6 achaete scute atonal amos Proneural genes Sensory organs + associated MD neurons solo-MD neurons

7 ESMD / mono-ESMD / poly-ES CHMD / CH solo-MD ASC ato amos Brewster and Bodmer, 1995 ES CH MD amos

8

9

10 Control of dendrite development Luo. et al. Trends Neuroscience (2003)

11 DA Dendrite formation

12 Constitutive active CDC42 induce supernumerary primary dendrites that fail to extend dorsally and grow secondary dendrites WT CDC42 pros mutations causes routing defect

13 Lateral branch defects: (A) WT, (B) reduced, (C) fail to extend, (E, F) over shrinking Increased density

14 Sequotia ( 水杉 ), Zn finger protein

15 No repulsion in dorsal group No invasion into ablated region in dorsal region in third instar

16 Competition between two dorsal groups of contralateral dorsal groups

17 Dorsal dendrite overextension in flamingo mutants

18 Class I Class II

19 Class III

20 Development (2002) Grueber et al Tiling of dendrites within the same class of DA neurons

21 Cell (2003) Grueber et al

22 Dendrites of different classes overlap extensively Class III and IVClass I and IV

23 Class IV tiling

24 Differential Cut levels in four types of DA neurons

25 cut mutant phenotypes in class III

26 Class II Class I

27 Class II

28 Class IV No effect on class III

29 Dendrites over-branching and over-lapping phenotypes

30 Defect in like-dendrite avoidance response WT fry

31 Heteroneuronal tiling defect Trc kinase Rac Fry branching tiling


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