1. The Egg

2. Egg Microvilli

3. Zona Pellucida Zp

4. Zona Pellucida Glycoproteins

5. The Sperm

6. Axonemes and Sliding Microtubules

7. Sperm Surface Asymmetry

8. Sea Urchins

9. Sea Urchin Acrosome Reaction

10. Sea Urchin Fertilization Direct Binding of Sperm to Egg

11. Competition Assay to Identify Egg Receptor

12. Isolated Zona Pellucida Blocks Sperm Binding + ZP (2 cell)

13. Isolated ZP3 Blocks Sperm Binding

14. Man NAcGlc O-linked Oligosaccharide NAcGlc Man NAcGlc O-linked Oligosaccharide NAcGlc Gal + 2UDP-Gal Galactosyl Transferase Galactosyl Transferase

15. Role of Galactosyl Transferase in Binding to Egg

16. Acrosome Reaction & Penetration of Zona Pellucida

17. Vesiculation of Acrosome

18. Fertilins and ADAMs family proteins

19. Fertilin-  hydrophobic domain

20. Model for Fertilin Function During Fertilization

21. Incorporation of Sperm Into Egg – Role of Microvilli

22. Incorporation of Sperm Components Into Egg

23. Formation of Sperm Pronucleus

24. Persistence of Sperm Mitochondria In Embryos

25. Propagation of Calcium “Wave”