1. DNA Laboratory SUNY College at Oneonta Dr. Nancy Bachman

2. Cells The ultimate microscale computers The ultimate microscale computers Different parts have special functions Different parts have special functions –Nucleus:heredity –Mitochondria:energy

3. DNA: Deoxyribonucleic Acid Found in cell nucleus Found in cell nucleus Provides the blueprint for proteins Provides the blueprint for proteins Order of bases specifies the code Order of bases specifies the code

4. Nucleotides Subunits of DNA Subunits of DNA = Sugar +phosphate +base = Sugar +phosphate +base Bases are Bases are A, C, G, T A, C, G, T

5. Laboratory methods DNA purification DNA purification PCR (polymerase chain reaction) PCR (polymerase chain reaction) Gel electrophoresis Gel electrophoresis DNA sequencing DNA sequencing

6. Applications Forensics Forensics Genetic Diseases Genetic Diseases Cell/Molecular Biology Research Cell/Molecular Biology Research Biocomputing Biocomputing

7. DNA isolation

8. PCR

9. DNA sequencing

10. Luminescence

11. Fluorescence

12. Fluorescent microscopy