1. Ectomycorrhizal dynamics of white fir stands at Teakettle A.D. Izzo and T.D. Bruns Plant and Microbial Biology University of California, Berkeley

3. Questions what is the community structure of ectomycorrhizal fungi associated with white fir? how does species richness and diversity change following fire/thinning? what is the role of the spore bank in these scenarios? how does the spore bank respond to heat/ash?

4. Sampling 0 51020 White fir stand

5. Techniques : RFLP matching ID of root tips by RFLP matches separate morphotypes freeze dry, weigh PCR RFLP DNA sequence (ITS) PCR RFLP match Species ID Taxonomic ID (Phylum, “Family”, Genus, RFLP taxon) ITS1F ITS4

6. ECM biomass highly variable

7. Agaricales Russulaceae Cortinariaceae PezizalesOtideaceaeWilcoxina Cortinarius Russula Cortinarioid1 Russuloid1 Thelephoroid2 Cencococcum Wilcoxina1 BK AB Basidio18 Basidiomycete Ascomycete Cencococcum3 Piloderma2Piloderma RFLP-taxon

8. Note minimal temporal similarity

9. Comparison of treatments Minimal overlap of dominant RFLP-taxa

10. Family-level comparison TK99TK01/99 (2 yrs)

12. 1 2 3 ECM “islands of dominance”

13. Additional studies Spatial bioassays - soils from all 54 plots pre-treatment - ABCO, PIJE, ABCO + PIJE Heat and Ash bioassays Pooled soil from 7-8 mature white fir stands across Teakettle C, 40, 55, 70 deg C, ash ABCO, PIJE

14. Acknowledgements Collectors 1999/2001 Martin Bidartondo Ryan Bowman Francisco Camacho Lisa Grubisha Erik Lilleskov Molecular analysis Josephine Agbowo Bioassay setup Kari Cheng Funding USDA, PMB dept