1. RAPID ALCOHOL TESTING IN WHOLE BLOOD BY DISK-BASED REAL-TIME ABSORPTION MEASUREMENT J. Steigert, L. Riegger, M. Grumann, T. Brenner, J. Harter, R. Zengerle, and J. Ducr é e IMTEK – University of Freiburg, Laboratory for MEMS Applications Georges-Koehler-Allee 106, D-79110 Freiburg, Germany (μTAS2005 P.1~3) Reporter: Chung-Kuang Chin( 秦重光 ) Date:11/28/2007

2. Outline Introduction Design & Theory Results Conclusion

3. Introduction routines in clinical diagnostics (resource consumption) －＞ compact point-of-care devices －＞ lab-on-a-chip (Bio-MEMS) disk-based, colorimetric alcohol assay & optical beam-guidance integrated system to rapidly determine the concentration of alcohol in human whole blood Lab on a disk －＞

4. Design & Theory (1) Disk-based platform － various microfluidic functions － polymer (COC) disk (bio-compatible) － high-throughput screening (HTS) Ref: www.answers.com/topic/data-recoverywww.answers.com/topic/data-recovery

5. Design & Theory (2) Optical path setup － total internal reflection (TIR) － extend optical path ( increase absorption )

6. Design & Theory (2) Total internal reflection (TIR)

7. Design & Theory (2) extend optical path － law of Beer-Lambert

8. Design & Theory (3) ☆ ALCOHOL ASSAY Vs = 500 nl Vr = 99.5 μl C.V. (sample metering) = 1.7 %

9. Design & Theory (3) ☆ ALCOHOL ASSAY

10. Results The performance is comparable to common breath analyzers

11. Conclusion This paper introduced a highly process integrated disk- based assay to rapidly and accurately determine the concentration of alcohol in human whole blood The assay is run on a cost efficient modular platform comprising a passive polymer disk with monolithically embedded optical and fluidic structures as well as a standard laser diode and detector Its high degree of automation, its short time-to-result and its excellent precision recommend our centrifugal platform for point-of-care testing and routine screening in clinical labs

12. THE END Thanks for Your Attention!

13. Reference M. Grumann et al., Proc. of MEMS 2005 conf., pp. 108–112, (2005) Jitae Kim,a Seh Hee Jang,b Guangyao Jia,a Jim V. Zoval,a Nancy A. Da Silvab and Marc J. Madou*a, Cell lysis on a microfluidic CD (compact disc),Lab on a chip, pp.516-522, (2004)