1. Antioxidative Fullerol for Orthopaedic Diseases
Xinlin Yang, PhD
Assistant Professor of Orthopaedics
May 9, 2015

2. Research interests Fullerene C60 and orthopaedic diseases
Fullerene C60 and cell differentiation/function
Yang X, Li CJ, Wan Y, Smith P, Shang G, Cui Q. Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells. Int J Nanomedicine Aug 20;9:
Yang X, Jin L, Yao L, Shen FH, Shimer AL, Li X. Antioxidative nanofullerol prevents intervertebral disk degeneration. Int J Nanomedicine May 15;9:
Yang X, Ebrahimi A, Li J, Cui Q. Fullerene-biomolecule conjugates and their biomedicinal applications. Int J Nanomedicine. 2014;9: Review.
Liu H, Yang X, Zhang Y, Dighe A, Li X, Cui Q. Fullerol antagonizes dexamethasone-induced oxidative stress and adipogenesis while enhancing osteogenesis in a cloned bone marrow mesenchymal stem cell. J Orthop Res Jul;30(7):
Yang X, Wan Y, Qiao X, Arlet V, Li X. Transcriptional alteration of matrix-related gene expression in cultured human disc cells by nanoparticles of a bismethanophosphonate fullerene. Cell Biol Int Aug;34(8):

3. Fullerene C60 Fullerene-ROS interactions
(Satoh and Takayanagi, 2006)
Drs. Smalley, Curl and Kroto, 1985/1996
Molecular model of Fullerene C60
Its antioxidative capacity hundreds times higher than…

4. Orthopaedic diseases/OS
Multifactorial diseases
Oxidative stress (OS)
(Hendrickx et al., 2015)

5. OS/adipo/osteo/pathways
Oxidative stress Adipogenesis
Oxidative stress Osteogenesis
(Atashi et al., 2015)

6. OS/OC/inflammation/pathways
Osteoclastogenesis
(Kanzaki et al., 2013)
Inflammation
(Emre et al., 2007)
Oxidative stress Osteoclastogenesis
Oxidative stress Inflammation

7. Biodistribution/Blood clearance
rabbit
TC99m-Fullerol
SPECT/
γ counter
Retained in bone marrow for up to 48 h
Half time of 1.9 h in blood
T1/2alpha =1.9 h

8. Osteonecrosis ROS Osteo Adipo Pending histological data of FH
D1 & hBMSC
Fullerol
ARS staining
ORO staning
qRT-PCR
Rabbit
MPSL
H & E staining
ROS
Osteo
Adipo
Pending histological data of FH

9. Osteoporosis RAW264.7 RANKL Fullerol Trap staining qRT-PCR Rabbit MPSL
Micro-CT OC
VB Bone loss

10. Disc degeneration RAW264.7 LPS Fullerol qRT-PCR ELISA Rabbit MPSL
Micro-CT
Inflammation
IVDD

11. Summary Fullerol is enriched in the femoral bone marrow.
Fullerol regulates differentiation of bone marrow stem cells and monocytic progenitors.
Fullerene as a powerful antioxidant could potentially be used for treatment of osteonecrosis, osteoporosis and intervertebral disc degeneration.

12. More orthopaedic diseases
Future Plans
Drug
More orthopaedic diseases
Fullerene
Fullerene A. B.
for bone marrow disorders C.
Our R21 on the topic of fullerene-PLGA for bone repair was scored 22. Release profile is being prepared.
Biomaterial
Scaffold
Fullerene
Novel antioxidative biomaterials

13. Funding Sources
Orthopaedic Research and Education Foundation/Zachary B. Friedenberg Clinician Scientist Award (Dr. Cui)
NIH/NIAMS R21 AR (Dr. Cui)