1. Senior Design P09052 Molecular Imaging System Upgrade Ben McGee Brandon Luquette Aaron Phipps Patricia Heneka Dien Nguyen

2. Project Description To develop a research prototype of a clinical system capable of performing depth-resolved optical molecular imaging of a stationary human hand – Integration of Qioptiq Linos’s Optigrid with existing Carestream molecular imaging platform. – Prototype must be functional and flexible enough for Carestream to investigate for commercialization

3. Proposed Modifications Existing Platform Prototype System

4. Optigrid Functionality Motors provide 120 o phase shifts and focus – Grid moves on the x-axis 11.11 um to complete a phase change – Improves Clarity – Grid moves 2 mm on the z-axis to focus image – 30 line pair/mm on a 10x10 mm square – Lines spatially modulate the illumination  A portion of the OptiGrid at 200x y xz

5. Design Objective Projection of the Optigrid at an angle while maintaining focus

6. Mechanical Design Solution

7. Implemented Mechanical Design

8. System Analysis and Calibration Characterization – Capable of projecting grid at spatial frequencies ranging from 0.8 – 1.4 line pair/mm Calibration – Developed a system specific procedure to provide repeatable phase movements Processing – Depth discrimination proof of concept using depth of modulation analysis

9. System Characterization.8 line pair/mm 1.4 line pair/mm Projected at a 30 o angle  Steepest angle capable of the system  The system was set to a zoom of 40 pixels/mm Magnified using Computar C mount lens  Different Lenses provide different spatial frequency ranges Captured with a blue excitation filter and a green emission filter

10. Calibration Coarse Calibration – Capture images in a general range of 30, 55 and 90 V – Calculate phase at each point – Determine approximate voltages for 120 o phase shifts.

11. Demodulation Images are combined to eliminate the projected frequency using Incorrectly phase shifted images have residual lines

12. Processing Phase shifted images were captured of the phantom block – Fluorescent sheets were inserted between polycarbonate sheets The block was imaged at two spatial frequencies

13. Depth Discrimination

14. Squared error away from the mean at each point is used to evaluate the depth at each point.

15. Recommendations Projection Angle – A shorter rail will result in a steeper projection angle which would reduce distortion Automation – The calibration process is very time consuming and prone to human error. – Timing is very critical to achieving repeatable phase shifts in the Optigrid. Calibration Procedure Modifications – Warm up routine – Increase the number of phase shifts

16. Thanks! Dr. Gilbert Feke Linda Antos Dr. Daniel Phillips Dr. Maria Helguera Carestream Health Qioptiq Linos Imaging Solutions