1. 1 kb ladderpBS uncutpBS BamH1pBS HindIIIpBS BamH1-HindIIIpCAT BamH1-HindIIIpCAT BamH1pCAT HindIIIpCAT uncutpCAT BamH1-HindIII partial digestpCAT BamH1-HindIII-EcoR1 3.0 kb 2.0 kb Note the partial digest has the linearized plasmid, the plasmid backbone and the CAT fragment CAT Plasmid backbone CAT fragment Control Gel

2. Tuesday lab gel 1 4123311 1 kb ladder 3.0 kb 2.0 kb Emily/RosarioDJMelanie/Laura 422 CAT fragment Vector fragment

3. Tuesday Lab Gel 2 1 kb ladder Elizabeth/ Vidi/Pavani Susan/ Aleks/ Laura Biff/Althea Adam/Meredith 1234 3.0 kb 2.0 kb 1234 12341234 Vector fragment CAT fragment

4. Wednesday Lab Gel 1 1 kb ladder EB/MBASC/DD SQ/TTDS/BAM 1 234 3.0 kb 2.0 kb 567891011 121314151617 CAT fragment Vector fragment 1-4 EB/MB: BS cut, CAT cut, BS uncut, CAT uncut 5-8 ASC/DD: BS cut, BS uncut, CAT cut, CAT uncut 9-13 SQ/TT: BS uncut, BS cut, CAT uncut, empty well, CAT cut 14-17 DS/BAM: BS cut, BS uncut, CAT cut, CAT uncut Marker

5. Wednesday Lab Gel 2 1 kb ladder DCP/WR SJK/FMM AW/JG/AF AW/AB 12 3 4 3.0 kb 2.0 kb 5678 91011 12 13141516 CAT fragment Vector fragment 1-4 DCP/WR: CAT cut, CAT uncut, BS cut, BS uncut 5-8 SJK/FMM: BS cut, BS uncut, CAT cut, CAT uncut 9-12 AW/JG/AF: BS uncut, BS cut, CAT uncut, CAT cut 13-16 AW/AB: CAT cut, CAT uncut, BS cut, BS uncut