1. 1.Which is the most unlikely involved in the intron- splicing of pre-mRNA ? [a. U1 RNP; b. 5' cap structure; c. polyadenylation signal; d. secondary structure of pre-mRNA], give a brief explanation if you are not sure your answer (5%)

2. U1A protein inhibits polyadenylation of its pre-mRNA U1A protein binds U1snRNA: 7-base sequence-U1A binding site

3. U1A protein inhibits polyadenylation of its pre-mRNA U1A binding site 2 copies in its own mRNA, at 3' end near the poly(A) signal prevent polyadenylation, but not the cleavage of pre-mRNA rapidly degraded

4. U1A protein inhibits polyadenylation of its pre-mRNA - prevent polyadenylation, but not the cleavage of pre-mRNA truncated mRNA without poly(A) tail, rapidly degraded

6. apo-B (apolipoprotein B), serum protein of lipid transporter, CAA  UAA, glutamine  stop codon, in intestine both liver and intestine forms transport lipid only liver apo-B delivers cholesterol containing LDL RNA editing in mammalian

7. Na +, Ca +2 ion channel, learning and memory CAG  CIG, glutamate  arginine, Ca +2 cannot pass Both editing of apo-B and glutamate receptor by RNA deaminases RNA editing in glutamate receptor mRNA

8. Iron dependent regulation of TfR mRNA stability. when iron ion high, IRE -BP inactive, cannot bind IRE, TfR mRNA is protected from degradation; when iron is low, IRE-BP is active and binds IRE. then TfR mRNA stability , TfR mRNA degraded

9. Iron dependent regulation of translation of ferritin stability. ferritin is an intracellular protein that binds iron ions ferritin, 5' IRE free of BP, translation occurs, more ferritin. The IRE in ferritin mRNA has no AU rich

10. ferritin

11. Using Yeast to study Eukaryotic Gene Function from Recombinant DNA (J Waston et al.) Ch 13 Size of genome in the selected organisms

12. Cloning yeast biosynthetic genes by complementation in E.coli Yeast biosynthetic genes are cloned by complementation of E.coli mutations

14. Classes of yeast vectors Shuttle vectors replicate in both E.coli and yeast

15. Cloning a yeast gene by complementation Yeast genes can be cloned by simple complementation

16. Generation of temperature sensitive mutants of yeast

17. Replica plating

18. Gene targeting by homologous recombination Homolgous recombination is a relative frequent event in yeast

19. Replacing a gene by transplacement

22. Tetrad analysis

23. Cloning genes required for mating reveals a signaling pathway similar to that seen in higher organisms Pheromone signaling pathway

24. Cloning of the GPA1 gene as a high-copy suppressor of pheromone sensitivity This fragment DNA only works in high copies, suggesting it was acting as a suppressor

26. The receptor swap experiment Genetic experiments in yeast can answer precise biochemical questions

27. U2 RNA base-pairs with an intron sequence

28. Base pairing between U2 and branch point (in yeast)

29. A genetic assay for protein-protein interactions

30. Plasmid shuffle Genetic analysis in yeast can be exploited to identify and study genes from higher organisms