1. Safe Working Practices for Honours, Directed Studies Students

2. General Safety Precautions General Rules  Lab Coat  Footwear  Handwashing  No Eating in Lab Area  Personal Belongings  Fire Procedure  Injuries  Emergency Number General Rules  Lab Coat  Footwear  Handwashing  No Eating in Lab Area  Personal Belongings  Fire Procedure  Injuries  Emergency Number

3. Biohazardous Materials Do not remove plates etc from lab Waste plates Waste non disposals Spills Nothing Goes Down The SINK Unprocessed Disinfect work area Spill cleanup Wash hands before leaving Do not remove plates etc from lab Waste plates Waste non disposals Spills Nothing Goes Down The SINK Unprocessed Disinfect work area Spill cleanup Wash hands before leaving

4. Chemical Preparation Check MSDS for proper handling Labeling and storage PPE Order needed Safe Disposal Check MSDS for proper handling Labeling and storage PPE Order needed Safe Disposal

5. Disposal Glassware Broken glass Autoclaving Hazardous wastes Glassware Broken glass Autoclaving Hazardous wastes

6. Glassware Rinse and ensure glassware is immersed in water in bucket in washup area When necessary do a load in dishwasher Return to appropriate shelf etc Rinse and ensure glassware is immersed in water in bucket in washup area When necessary do a load in dishwasher Return to appropriate shelf etc

7. Broken Glass Not contaminated with biohazard in glass breakage containers Biohazard contaminated: yellow sharps containers Leave note on white board in S365C if broken glassware Not contaminated with biohazard in glass breakage containers Biohazard contaminated: yellow sharps containers Leave note on white board in S365C if broken glassware

8. Autoclaving Waste biohazards Liquids –Use slow exhaust Wrapped –Allow for drying cycle Autoclave tape Check with others before starting (particularly Carolynne) Waste biohazards Liquids –Use slow exhaust Wrapped –Allow for drying cycle Autoclave tape Check with others before starting (particularly Carolynne)

9. Hazardous Waste Container –Appropriate size Labeling –Contains all ingredients (no abbreviations) Location –S365F Container –Appropriate size Labeling –Contains all ingredients (no abbreviations) Location –S365F

10. Before Leaving After Every Session Clean any areas that have been used (balances, counter, fumehood) Glassware is put in washup area Turn off gas Turn off deionized water Turn off lights Turn off autoclave Lock door Clean any areas that have been used (balances, counter, fumehood) Glassware is put in washup area Turn off gas Turn off deionized water Turn off lights Turn off autoclave Lock door

11. Working Alone If at all possible avoid it A risk assessment of your work must be done with your supervisor to determine what can be done with minimum supervision Higher risk procedures should not be done when alone (working with hazardous materials, risk of fire) http://www.ccohs.ca/oshanswers/hsprograms/ workingalone.html If at all possible avoid it A risk assessment of your work must be done with your supervisor to determine what can be done with minimum supervision Higher risk procedures should not be done when alone (working with hazardous materials, risk of fire) http://www.ccohs.ca/oshanswers/hsprograms/ workingalone.html

12. Working Alone The Faculty of Science will be striking a multidiscipline committee to develop working alone guidelines for the Faculty of Science Have some type of checkin procedure The Faculty of Science will be striking a multidiscipline committee to develop working alone guidelines for the Faculty of Science Have some type of checkin procedure

13. Safe Work Procedures http://www.tru.ca/hsafety/working learningsafely/work.htmlhttp://www.tru.ca/hsafety/working learningsafely/work.html http://www.tru.ca/hsafety/working learningsafely/work.htmlhttp://www.tru.ca/hsafety/working learningsafely/work.html

14. WHMIS

15. What is WHMIS Workplace Hazardous Materials Information System Provides information about hazardous materials used in the workplace Workers have the right to receive information about each controlled product they use Goal of WHMIS is to reduce injury and disease. Workplace Hazardous Materials Information System Provides information about hazardous materials used in the workplace Workers have the right to receive information about each controlled product they use Goal of WHMIS is to reduce injury and disease.

16. HAZARD CLASSES 1.Class A: Compressed Gas 2.Class B: Flammable & Combustible 3.Class C: Oxidizing Material 4.Class D: Poisonous & Infectious Material 1.Class A: Compressed Gas 2.Class B: Flammable & Combustible 3.Class C: Oxidizing Material 4.Class D: Poisonous & Infectious Material

17. HAZARD CLASSES Class E: Corrosive Material Class F: Dangerously Reactive Material Class E: Corrosive Material Class F: Dangerously Reactive Material

18. SUPPLIER LABELS Seven items of information must be included on supplier label: 1.Product identifier 2.Hazard Symbols 3.Risk Phrase 4.Precautionary Statements 5.First Aid Measures 6.Supplier Identification 7.Reference to MSDS Seven items of information must be included on supplier label: 1.Product identifier 2.Hazard Symbols 3.Risk Phrase 4.Precautionary Statements 5.First Aid Measures 6.Supplier Identification 7.Reference to MSDS

20. WORKPLACE LABELS Required on containers for each controlled product on site, including secondary containers. Provide 3 types of information: 1.Product Identifier 2.Safe Handling and personal protective clothing and equipment. 3.Reference to MSDS Required on containers for each controlled product on site, including secondary containers. Provide 3 types of information: 1.Product Identifier 2.Safe Handling and personal protective clothing and equipment. 3.Reference to MSDS

21. WORKPLACE LABEL

22. MSDS SHEET Material Safety Data Sheet provides specific hazard information, safe handling information, and emergency procedures for a controlled product. Key source of information for developing safe working procedures. Material Safety Data Sheet provides specific hazard information, safe handling information, and emergency procedures for a controlled product. Key source of information for developing safe working procedures.

23. MSDS SHEETS Nine Recommended Section Headings: 1.Product Information 2.Hazardous Ingredients 3.Physical Data 4.Fire and Explosion Hazard 5.Reactivity Data 6.Toxicological Properties 7.Preventative Measures 8.First Aid Measures 9.Preparation Information Nine Recommended Section Headings: 1.Product Information 2.Hazardous Ingredients 3.Physical Data 4.Fire and Explosion Hazard 5.Reactivity Data 6.Toxicological Properties 7.Preventative Measures 8.First Aid Measures 9.Preparation Information

24. Cycloheximide MSDS Routes of Entry: Absorbed through skin. Eye contact. Inhalation. Ingestion. Toxicity to Animals: Acute oral toxicity (LD50): 2 mg/kg [Rat]. Chronic Effects on Humans: DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Development toxin [POSSIBLE]. Causes damage to the following organs: the nervous system, liver. Routes of Entry: Absorbed through skin. Eye contact. Inhalation. Ingestion. Toxicity to Animals: Acute oral toxicity (LD50): 2 mg/kg [Rat]. Chronic Effects on Humans: DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Development toxin [POSSIBLE]. Causes damage to the following organs: the nervous system, liver.

25. Cycloheximide May cause damage to the following organs: blood, kidneys, gastrointestinal tract, skin. Other Toxic Effects on Humans: Very hazardous in case of skin contact (irritant), of ingestion, of inhalation. May cause damage to the following organs: blood, kidneys, gastrointestinal tract, skin. Other Toxic Effects on Humans: Very hazardous in case of skin contact (irritant), of ingestion, of inhalation.

26. TRU MSDS Bookmark student computer S365B ThompsonMSDS Msds Read before using new chemicals Bookmark student computer S365B ThompsonMSDS Msds Read before using new chemicals

27. RESPONSIBLITIES Workers 1.Know & understand info on labels and MSDS 2.Handle controlled products safely. 3.Inform employer if labels missing Employer 1.Ensure workers understand WHMIS 2.Provide training 3.Ensure controlled products have MSDS and WHMIS Labels 4.MSDS available to workers Workers 1.Know & understand info on labels and MSDS 2.Handle controlled products safely. 3.Inform employer if labels missing Employer 1.Ensure workers understand WHMIS 2.Provide training 3.Ensure controlled products have MSDS and WHMIS Labels 4.MSDS available to workers