1. Normal Flora Microbiotica

4. Synder Deep CH2O fermentation –Glucose Differential –Rate of change –susceptibility Selective for lactobacillus –pH = 4.8 –Indicator = bromcresol green –Dental caries

5. BAP Divide Plate Swab throat Differential –Sheep Blood –Hemolysis Hemolysin exotoxins Types –Gamma –Alpha –Beta

6. Chocolate Agar Divide Plate Swab throat Differential –Growth –Oxidase test Neisseria Lysed RBCs

7. MSA Selective Differential Divide plate Swab Skin –Nose –Face Results –Fermentation –acid end products –yellow Components –7.5% NaCl –mannitol –pH indicator = phenol red

8. MH-T Divide plate Swab skin –Ears –Palms Differential –Diptheroids Tellurite –Reduced to tellurium –Black (grey) colonies

9. Nutrient Agar TSA –Not selective –Not differential Divide plate Swab environment –Moisten swab –Label source

10. Sabourauds Selective –Yeast –Fungus +/- Differential Acidic pH Divide plate Swab environment –Moisten swab –Label source

11. Urine Culture TSA plate Calibrated loop (0.01ml) Clean catch sample Swab plate –Confluent –Isolation Calculate CFU/ml

12. Normal Flora Results

13. Snyder Deep Results CH2O fermentation –Glucose Differential –Rate of change –Susceptibility for caries Selective for lactobacillus –pH = 4.8 –Indicator = bromcresol green –Green  Yellow –Dental caries Slight Mild Moderate Severe

14. BAP results Not selective Differential Hemolysis –Hemolysin exotoxins –Types Gamma Alpha Beta

15. Chocolate Agar Results Differential –Growth –Lysed RBCs –CO2 = capnophiles Oxidase Test –Neisseria

16. Oxidase Test Oxidase Reagent –Cytochrome C in ETC –Dark Purple = positive –Neisseria

17. Oxidase Reaction

18. MSA Results Selective Differential Results –Fermentation –acid end products –yellow Components –7.5% NaCl –Mannitol –pH indicator = phenol red

19. MH-T Results Differential –Diptheroids Mueller Hinton –Nutrient agar –Controlled proteins/ions –Diffusion Tellurite –Reduced to tellurium –Black (grey) colonies

20. Sabarouds Results Selective –Yeast –Fungus +/- Differential Acidic pH –5.5 –acidophiles

21. TSA Environmental Culture

22. UTI CFU/ml # colonies/0.01ml UTI –= > 1x10 (5)/ ml –Clinical signs –Other urine tests