1. Ozone Testing on Honey Bee EquipmentBy
Elmer James
Martin James
Karla James Shelton Of
Slide Ridge Honey
Mendon, Utah
June 9, 2008

2. Assisted by: O3 Company, Aberdeen, Idaho
USDA-ARS Bee Research Lab, Beltsville
Utah Dept. of Agriculture, Salt Lake City
USDA-ARS Pollinating Insect Research Unit, Logan, Utah
Ozone Research Equipment, Akron, Ohio

3. Lab Facilities
Small Test Chamber at Ozone Research Equipment in Akron, Ohio
Lab Testing at USDA-ARS Beltsville Bee Lab in Beltsville, Maryland.
Lab Testing at Utah Dept of Agriculture in Salt Lake City, Utah
Lab Assistance from USDA-ARS Bee Lab in Logan, Utah.

4. Full Scale Field Testing
Ozone room constructed at Slide Ridge Honey in Mendon, Utah
Size: 20 ft. X 15 ft. X 10 ft.
Large ozone generator unit , double chamber type manufactured by O3 company in Aberdeen, Idaho.
Monitor with range 0 to 2000 PPM provided by O3 company.

5. Ozone Room

6. Ozone Room Sealed

7. Ozone Generator

8. Ozone Generator Inside

9. Ozone Monitor

10. Ozone Test Start up Number 1

11. Ozone Test Start up Number 2

12. Ozone Test Start up Number 3

13. Ozone Test Start up Number 4

14. Ozone Test Run Number 2

15. Ozone Test Run Number 3

16. Ozone Test Run Number 4

17. Part of Ozone Test Run Number 4 (USU Data Logger)

18. Ozone Test Run Number 5

19. Ozone Test Run Number 5 (USU Data Logger Record)

20. Observations Pesticides residue destroyed.
Herbicides residue destroyed.
Wax moth destroyed, inactive remains.
Gasoline residue destroyed on outer surface during run #5 on a customer’s Hives.
Comb readily accepted by Honey Bees.
Dry Rot fungus may be stopped.

21. Observations Con’t.
Foundation purchased several years ago was never acceptable to our Honey Bees. Now that this foundation has been submitted to Ozone they seem to readily move on to it and start building comb.

22. Items not Subdued FOULBROOD
More testing required & with different techniques.

23. State of Utah Lab Tests
Control Sample of Beeswax & Pollen with no Pesticides applied after Ozone treatment.
Beeswax & Pollen with Fluvalinate applied and then treated with Ozone.
Beeswax & Pollen with Amitraz applied and then treated with Ozone.

24. State of Utah Sample

25. State of Utah Sample

26. State of Utah Sample

27. Wax Moth Damage
Comb with severe wax moth damage has never been acceptable to our Honey Bees, therefore these frames were disposed of in our operation.
Same severe damaged comb, Ozone treated, is very acceptable, the bees move right on to it and starting cleaning out the cells for reuse. Refuse dumped on bottom board by the bees, tip hive and scrap out.

28. Typical Wax Moth Damage

29. Typical Damaged Frame After Ozone Treatment

30. Drying out Equipment
The Ozone process seems to dry out all of the equipment, boxes, frames, comb, pallets, etc.
O3 CO theorizes that dry air input as the carrier for the Ozone gas is absorbing the moisture from the equipment.

31. Room Pressure Exhaust With Charcoal Filter

32. Air Circulation In Ozone Room, NO Rubber or Urethane Belts

33. Air Circulation in Ozone Room Requires Direct Drive Blower

34. Coding of Equipment Processed Through Ozone

35. Coded Hive Box & Frames

36. Restack Hive Boxes to Allow Better Air Circulation

37. Locate Ozone Sensor Near Top of Equipment Being Processed

38. Depressurize ducting, route outside of building

39. Cost Ozone generator $3,000.00 per month lease.
Electric power $
Frames processed 8,880.
Labor $0.00.
Cost per brood frame = $0.36 each.
Not included, improvement to boxes, pallets, lids, etc.

40. End Result of Ozone Treated Equipment. (30 Day Nuc)

41. Honey Bee Care
Bees shown in slides have been treated for Mites, Nosema, Foulbrood, and Chalkbrood.
Their diet has been supplemented with Bee-Pro & other additives.

42. Conclusions by Slide Ridge Honey
All “Dead Out Hives” should be processed in an Ozone Chamber before attempting to raise Honey Bees in them.
We consider placing Honey Bees in unprocessed “Dead Out Hives”, a waste of time and money.

43. Conclusions Continued
Why does Ozone Treatment Work?
This was the first full scale Ozone Test under field conditions, and we know a number of items that are being destroyed, however we may have wiped out other detrimental elements that need to be known and requires further Lab Research.

44. Comments
We wish to acknowledge the USU Bee Lab and their staff for coming up with the idea of using Ozone to purify Bee Hives, and now that we have proven that the idea is economically feasible by Bee Keepers at the commercial level, we encourage USU Bee Lab to enlarge this research program at the earliest opportunity.

45. Comments Continued
We believe that with Ozone Processing and proper nutrition, CCD may disappear from the Bee Keeping operation.
We believe that our two biggest problems in bee keeping are: pesticides/herbicides being returned to the hive & poor honey bee nutrition.

46. Factor We need for the Lab to establish a factor that relates:
Time in Ozone chamber to PPM of Ozone
This would allow us to cut cost on power consumption and/or equipment size.
We are also of the opinion that the Logan Bee Lab is in the best position to conduct this Research.

47. Distribution State of Utah Dept. of Agriculture & Food
USDA-ARS Bee Lab
5310 Old Main Hill
Room 253
Logan, Utah
Dr. Rosalind James
Craig Huntzinger
Ellen Klinger
State of Utah
Dept. of Agriculture & Food
350 N. Redwood Road
Salt Lake City, Utah
Danielle Downey
Dr. David Clark

48. Distribution Con’t. Beltsville Bee Lab Room 211 10300 Baltimore AV.
Building 476 BARC-East
Beltsville, MD 20705
Bart Smith
Jeffery Pettis
O3 Manufacturing
2775 West 1800 South
Aberdeen, Idaho 83210
Lynn Johnson
cell

49. Distribution Con’t. Ozone Research Equipment Company 221 Beaver Street
Akron, Ohio 44304
Ron Brainard
Bee Keepers:
Newswander Brothers Honey
Pendell Apiaries
Homan McMaster Apiaries
Mike Brockman

50. Distribution Con’t. Suppliers: Mann Lake Ltd. Stuart Volby
501 1st. St. South
Hackensack, MN